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Spiroplasma citri

ABSTRACT: Causes citrus stubborn disease

PROVIDER: PRJNA35495 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR10507065_subreads.fastq.gz	Fastqsanger.gz
	SRR10507066_subreads.fastq.gz	Fastqsanger.gz
	SRR10507067_subreads.fastq.gz	Fastqsanger.gz
	SRR10507068_subreads.fastq.gz	Fastqsanger.gz

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Spiroplasma citri

Project description:The causative agent of Citrus Stubborn Disease

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Gene expression profiles of Washington navel oranges (Citrus sinensis (L) Osbeck) graft innoculated with Candidatus Liberibacter asiaticus, Spiroplasma citri, or two citrus tristeza isolates

Project description:Pathogens can trigger a broad array of changes in gene expression in plants. In this study we report the changes in gene expression patterns that occurred when greenhouse grown Washington Navel oranges (C. sinensis) was graft innoculated with citrus pathogens. Candidatus Liberibacter asiaticus, Spiroplasma citri, and two isolates of citrus tristeza virus were studied.

2021-07-31 | GSE167171 | GEO

Xanthomonas citri pv. citri str. 306

Project description:Plant-specific pathogen that causes citrus canker

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Characterization of the recA gene regions of Spiroplasma citri and Spiroplasma melliferum.

Project description:In previous studies (A. Marais, J. M. Bove, and J. Renaudin, J. Bacteriol. 178:862-870, 1996), we have shown that the recA gene of Spiroplasma citri R8A2 was restricted to the first 390 nucleotides of the N-terminal part. PCR amplification and sequencing studies of five additional strains of S. citri have revealed that these strains had the same organization at the recA region as the R8A2 strain. In contrast to S. citri, Spiroplasma melliferum was found to contain a full-length recA gene. However, in all five S. melliferum strains tested, a TAA stop codon was found within the N-terminal region of the recA reading frame. Our results suggest that S. melliferum, as well as S. citri, is RecA deficient. In agreement with the recA mutant genotype of S. citri and S. melliferum, we have shown that these organisms are highly sensitive to UV irradiation.

| S-EPMC178606 | biostudies-other

Spiroplasma kunkelii CR2-3x

Project description:Causes corn stunt disease

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Nucleotide sequence of the Spiroplasma citri fibril protein gene.

Project description:Electron microscopic observation of spiroplasmas lysed by detergent (sodium deoxycholate) revealed the release of bundles of fibrils from the cells. Individual fibrils are 4 nm in diameter and possess a 9-nm periodicity along their length. These fibrils are thought to function as cytoskeletal structures involved in the shape and motility of spiroplasmas. Polyacrylamide gel electrophoresis of density gradient-purified fibrils showed a protein of approximately 55 kDa. Oligonucleotide probes were constructed from the N-terminal amino acid sequence of two peptides obtained after V8 protease hydrolysis of the fibril protein. The probes were used to identify the clones in a genomic DNA library of Spiroplasma citri that contained inserts carrying the probe sequence. Sequencing of a 3.3-kbp fragment yielded the full open reading frame of the fibril protein gene and the start of a second open reading frame of an unknown protein. The fibril protein is composed of 515 amino acids, which have a computed molecular mass of 59 kDa. Northern (RNA) blot hybridization and primer extension experiments showed that transcription of the fibril protein gene starts from a promoter located 100 nucleotides upstream of the initiation codon and stops at a rho-independent type terminator, leading to a 1.7-kbp transcript. Southern blot hybridization of genomic DNA using the fibril protein gene as the probe showed that a single copy of the gene is present in the chromosomes of both S. citri and Spiroplasma melliferum. The genotypic symbol fib is proposed for the spiroplasma fibril protein gene.

| S-EPMC208096 | biostudies-other

Citrus limon: Control vs. Xanthomonas citri T2 inoculation

Project description:Transcriptional Profiling of non-inoculated Citrus limon leaves vs. 48h inoculation with Xanthomonas citri T2

2016-12-18 | GSE78012 | GEO

Citrus limon: Control vs. Xanthomonas citri AT inoculation

Project description:Transcriptional Profiling of non-inoculated Citrus limon leaves vs. 48h inoculation with Xanthomonas citri AT

2016-12-18 | GSE78010 | GEO

Spiroplasma citri

Project description:Spiroplasma citri Genome sequencing and assembly

| PRJNA591027 | ENA

Project description:pathogen that causes citrus disease

| PRJNA745208 | ENA