Pseudomonas aeruginosa
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA355866 | ENA |
REPOSITORIES: ENA
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Project description:Aspergillus fumigatus and Pseudomonas aeruginosa are the most prevalent fungal and bacterial pathogens, associated with cystic fibrosis-related infections, respectively. Although it is well established that P. aeruginosa eventually predominates as the primary pathogen, it is unknown why this is the case. Label-free quantitative proteomics was employed here to compare the cellular response to A. fumigatus, P. aeruginosa and sequential exposure to both pathogens in the type II alveolar epithelial cell line, A549, a model of the alveolar surface. In total, 2264 proteins were identified and the analysis of statistically significant (p<0.05; ±1.5 fold change), differentially abundant (SSDA) proteins, revealed an increase in the relative abundance of proteins associated with biological processes common to all pathogen-exposed groups. These included mitochondrial stress, energy metabolism and protein processing in the endoplasmic reticulum. Conversely, clear differences were observed between the cellular response to A. fumigatus or P. aeruginosa and following sequential exposure to both pathogens. The relative abundance of proteins associated with ribosomal activity appeared comparable between sequentially exposed cells and unexposed cells, but were substantially decreased in fungal- and bacterial-exposed cells. Furthermore, compared to unexposed cells, the relative abundance of proteins associated with the actin cytoskeleton, endocytosis, phagosome and lysosome were increased in A. fumigatus and P. aeruginosa-exposed cells, but appeared unchanged or decreased in sequentially exposed cells. This research provides novel insights into the whole cell proteomic response to A. fumigatus and P. aeruginosa, and highlights distinct differences following sequential exposure to both pathogens.
2019-11-11 | PXD014022 | Pride
Project description:Purpose: To determine effects of arsenic on gene expression in polarized primary human bronchial epithelial (HBE) cells and impact on transcriptional response to Pseudomonas aeruginosa infection Methods: mRNA profiles of HBE cells from 6 donors exposed to 0, 5, 10 or 50 ug/L total arsenic +/- Pseudomonas aeruginosa (48 samples) were generated using Illumina sequencing, aligned in CLC Genomics workbench and analyzed for DE in EdgeR Findings: 20-30 million reads were mapped per sample and transcripts were identifed that were significantly differentially expressed in response to arsenic and Pseudomonas aeruginosa
2017-06-26 | GSE97036 | GEO
Project description:We report RNA sequencing data for mRNA transcripts obtained from tobramycin exposed phoenix colonies, VBNCs, and various controls (untreated lawn, edge of the zone of clearance of tobramycin, treated outer background lawn). Extracted mRNA was sequenced using an Illumina HiSeq 4000, mapped to a Pseudomonas aeruginosa PAO1 reference genome, and processed to obtain counts for all gene transcripts for each sample. This is the first sequencing data generated for Pseudomonas aeruginosa phoenix colonies and VBNCs.
2022-03-27 | GSE199323 | GEO
Project description:Anthropogenic pollution has increased the levels of heavy metals in the environment. Bacterial populations continue to thrive in highly polluted environments and bacteria must have mechanisms to counter heavy metal stress. We chose to examine the response of the environmentally-relevant organism Pseudomonas aeruginosa to two different copper treatments. A short, 45 min exposure to copper was done in the Cu shock treatment to examine the immediate transcriptional profile to Cu stress. The Cu adapted treatment was designed to view the transcriptional profile of cells that were actively growing in the presence of Cu. Experiment Overall Design: We analyzed 2 biological replicates of Pseudomonas aeruginosa exposed to a 45 min Cu shock as compared to a control that was exposed to HCl to bring the pH to similar levels. We analyzed 2 biological replicates of Pseudomonas aeruginosa that were grown in the presence of Cu for approx. 6h (Cu adapted) as compared to an untreated control.
2007-10-24 | E-GEOD-4152 | biostudies-arrayexpress
Project description:Analysis of Pseudomonas aeruginosa PAO1 treated with 200 µM sphingomyelin. Results provide insight into the response to sphingomyelin in P. aeruginosa.
2017-02-12 | GSE83078 | GEO
Project description:To gain insights into the initial phases of P. aeruginosa infections and to identify P. aeruginosa genes regulated in response to respiratory epithelia we exposed P. aeruginosa to cultured primary differentiated human airway epithelia. We used a P. aeruginosa strain (PAO1) that causes acute damage to the epithelia and a mutant (PAOSC11) with defects in Type III secretion and in rhamnolipid synthesis. The mutant did not cause rapid damage to epithelia as did the wildtype. Keywords: Pseudomonas aeruginosa and respiratory epithelia
2006-12-19 | GSE4614 | GEO
Project description:In the present study, we employed Affymetrix Pseudomonas aeruginosa GeneChip arrays to investigate global gene expression profiles during the cellular response of Pseudomonas aeruginosa to sodium hypochlorite Keywords: Antimicrobial response
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Project description:We report the transcriptome of Pseudomonas aeruginosa PA14 under swarming conditions as compared to a swimming control
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Project description:This study addresses the impact of zinc limitation on the opportunistic human pathogen, Pseudomonas aeruginosa. Zinc limitation was assessed in the P. aeruginosa PAO1 strain using an isogenic deletion mutant lacking the periplasmic, zinc solute-binding protein, znuA (PA5498). ZnuA delivers bound zinc to its cognate ABC transporter, ZnuBC, for import into the cytoplasm. Our transcriptional analyses revealed P. aeruginosa to possess a multitude of zinc acquisition mechanisms, each of which were highly up-regulated in the zinc-deficient znuA mutant strain. P. aeruginosa also utilized zinc-independent paralogues of zinc-dependent genes to maintain cellular function under zinc limitation. Together, these data reveal the complex transcriptional response and versatility of P. aeruginosa to zinc depletion.
2017-01-01 | GSE60177 | GEO
Project description:Transcriptional profile of Pseudomonas aeruginosa infected cells
2023-01-01 | GSE199424 | GEO