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Homo sapiens

ABSTRACT: Expression data for hiPSC-derived RPE treated with 10mM Nicotinamide or vehicle

PROVIDER: PRJNA356283 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5077520.fastq.gz	Fastqsanger.gz
	SRR5077530.fastq.gz	Fastqsanger.gz
	SRR5077521.fastq.gz	Fastqsanger.gz
	SRR5077522.fastq.gz	Fastqsanger.gz
	SRR5077523.fastq.gz	Fastqsanger.gz

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Homo sapiens

Project description:Expression data for hiPSC-derived RPE treated with 10mM Nicotinamide or vehicle

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Expression data for hiPSC-derived RPE treated with 10mM Nicotinamide or vehicle

Project description:We did the RNA-seq analysis to examine the global impact of Nicotinamide (NAM) on hiPSC-derived RPE transcriptome in order to better understand the mechanism of action of NAM. NAM inhibited the expression of Age related Macular degeneration (AMD) associated protein transcripts in hiPSC-derived RPE.

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Expression data for hiPSC-derived RPE treated with 10mM Nicotinamide or vehicle

Project description:Nicotinamide (NAM) inhibited the expression of Age related macular degeneration (AMD) associated proteins in hiPSC-derived retinal pigment epithelium (RPE). We did the microarray analysis to examine the global impact of NAM on hiPSC-derived RPE transcriptome in order to better understand the mechanism of action of NAM.

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Project description:Expression data from human induced pluripotent stem cells derived retinal pigment epithelium (hiPSC-RPE)

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Project description:Transcriptomic analysis of corticosteroids-treated hiPSC-derived RPE cells

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The effect of nicotinamide on dysregulated genes associated with frataxin deficiency in FRDA.

Project description:To investigate the efficacy of nicotinamide treatment using our ex-vivo primary lymphocyte model, we performed high-throughput RNA sequencing on libraries generated from untreated and nicotinamide treated samples. PBMC isolated from FRDA affected individuals were cultured to prepare the primary lymphocyte cell lines. The primary cultured cells were either treated with 10mM nicotinamide or without the addition of drug during the 3-days treatment. RNA was extracted after the treatment and then RNA-seq libraries were generated by standard protocols.

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Project description:The goals of this study are to generate inflammation-sensitive astrocytes from human induced pluripotent stem cells. We examine the transcriptomic inflammatory signature of generated astrocytes following Il1Beta exposure. Primary human cerebellar astrocytes, human induced pluripotent stem cells (hiPSC)-derived neural precursor cells (NPCs) and hiPSC-derived astrocytes were treated with Il1beta and compared to vehicle treated controls. Results: hiPSC-derived can be differentiated to astrocytes that are inflammation sensitive.

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Crystal structure of Homo sapiens kynureninase.

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