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Mus musculus

ABSTRACT: Integrative analysis of sex-specific microRNA networks following stress in mouse nucleus accumbens

PROVIDER: PRJNA356453 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5082200_1.fastq.gz	Fastqsanger.gz
	SRR5082200_2.fastq.gz	Fastqsanger.gz
	SRR5082210.fastq.gz	Fastqsanger.gz
	SRR5082220.fastq.gz	Fastqsanger.gz
	SRR5082201_1.fastq.gz	Fastqsanger.gz

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Integrative analysis of sex-specific microRNA networks following stress in mouse nucleus accumbens

Project description:Adult women are twice as likely as men to suffer from affective and anxiety disorders, although the mechanisms underlying heightened female stress susceptibility are incompletely understood. Recent findings in mouse Nucleus Accumbens (NAc) suggest a role for DNA methyltransferase-driven sex differences in genome-wide transcriptional profiles. However, the role of another epigenetic process—microRNA (miR) regulation—has yet to be explored. We exposed male and female mice to Subchronic Variable Stress (SCVS), a stress paradigm that produces depression-like behavior in female, but not male, mice, and performed next-generation mRNA and miR sequencing on NAc tissue. We applied a combination of differential expression, miR-mRNA network and functional enrichment analyses to characterize the transcriptional and post-transcriptional landscape of sex differences in NAc stress response. We find that male and female mice exhibit largely non-overlapping miR and mRNA profiles following SCVS. The two sexes also show enrichment of different molecular pathways and functions. Collectively, our results suggest that males and females mount fundamentally different transcriptional and post-transcriptional responses to SCVS and engage sex-specific molecular processes following stress. These findings have implications for the pathophysiology and treatment of stress-related disorders in women.

2017-02-09 | GSE90962 | GEO

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Project description:Expression data from adult mouse Nucleus Accumbens following prenatal infection

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MicroRNA expression profile of methamphetamine-sensitized and saline-control mice examined by high throughput sequencing

Project description:Using microRNA-Seq, we examined the alteration of microRNA expression profiles in the nucleus accumbens of methamphetamine-sensitized and saline-control mice. Nucleus accumbens lysate from eight mice of each group were pooled as one sample. Two RNA samples from each group were prepared and were then processed to generate small RNA libraries, which were sequenced on the Illumina Hiseq 2000. The aberrant expression of microRNAs identified in this study may involve in METH-induced locomotor sensitization and addiction.

2022-07-13 | E-MTAB-2901 | biostudies-arrayexpress

Cell-type and sex-specific rhythmic gene expression in the nucleus accumbens

Project description:Cell-type and sex-specific rhythmic gene expression in the nucleus accumbens

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Adult male prairie vole nucleus accumbens RNAseq dataset for "Prolonged partner separation erodes nucleus accumbens transcriptional signatures of pair bonding in male prairie voles."

Project description:This dataset contains RNA sequencing results from adult male prairie voles that were in either opposite-sex or same-sex pairs that were subsequently either separated from or remained paired with their partner for either 48 hours or 4 weeks prior to collecting nucleus accumbens tissue. The goal of this experiment was to determine the nucleus accumbens transcriptional response specific to separation from an opposite-sex partner. RNA sequencing was done on polyA enriched transcripts using Illumina single-end sequencing. Samples from 3 groups were from a Ribo-seq protocol using a virally delievered, vole optimized Translating Ribosome Affinity Purification construct (Heiman et al., 2008). These samples contain files for both the input fraction and the pulldown fraction (denoted with a _P suffix).

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Sex Differences in Nucleus Accumbens Transcriptome Profiles Associated with Susceptibility versus Resilience to Subchronic Variable Stress

Project description:Depression and anxiety disorders are more prevalent in females, but the majority of research in animal models, the first step in finding new treatments, has focused predominantly on males. Here we report that exposure to subchronic variable stress (SCVS) induces depression-associated behaviors in female mice, whereas males are resilient as they do not develop these behavioral abnormalities. In concert with these different behavioral responses, transcriptional analysis of nucleus accumbens (NAc), a major brain reward region, by use of RNA sequencing (RNA-seq) revealed markedly different patterns of stress regulation of gene expression between the sexes. Among the genes displaying sex differences was DNA methyltransferase 3a (Dnmt3a), which shows a greater induction in females after SCVS. Interestingly, Dnmt3a expression levels were increased in the NAc of depressed humans, an effect seen in both males and females. Local overexpression of Dnmt3a in NAc rendered male mice more susceptible to SCVS, whereas Dnmt3a knock-out in this region rendered females more resilient, directly implicating this gene in stress responses. Associated with this enhanced resilience of female mice upon NAc knock-out of Dnmt3a was a partial shift of the NAc female transcriptome toward the male pattern after SCVS. These data indicate that males and females undergo different patterns of transcriptional regulation in response to stress and that a DNA methyltransferase in NAc contributes to sex differences in stress vulnerability. mRNA profiles of sex-specific in stress response were generated in the Nucleus Accumbens for 3 female control mice, 3 male control mice, 3 stressed female mice, 3 stressed male mice. Cre-mediated Dnmt3a knock-out was performed on stressed mice, with 3 males and 3 females expressing control GFP and 3 males and 3 females expressing CRE/GFP.

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2021-09-08 | PXD013146 | Pride

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Project description:Tet1 in nucleus accumbens regulates stress responses

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Expression data in the mouse brain following the glucocorticoid receptor overexpression in the forebrain (GRov) during different periods in development

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