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Escherichia coli str. K-12 substr. MG1655

ABSTRACT: ChIP-Seq experiment for the global regulators, CRP and Fis in early and mid exponential growth phases

PROVIDER: PRJNA357050 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5099120_1.fastq.gz	Fastqsanger.gz
	SRR5099120_2.fastq.gz	Fastqsanger.gz
	SRR5099130_1.fastq.gz	Fastqsanger.gz
	SRR5099130_2.fastq.gz	Fastqsanger.gz
	SRR5099140_1.fastq.gz	Fastqsanger.gz

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ChIP-Seq experiment for the global regulators, CRP and Fis in early and mid exponential growth phases

Project description:We have performed ChIP-Seq experiment for the global regulators, CRP and Fis in early and mid exponential growth phases respectively in Escherichia coli K12 MG1655. The dataset contains the genome wide binding patterns of Fis and CRP in the wildtype and the mutant strains

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Project description:Comparison of transcript profiles of E. coli CSH50 wild-type and fis or hns mutant cells to distinguish between the genes expressed either in the presence or absence of transcriptional regulators FIS and H-NS during different growth phases. Samples were taken in Mid-exponential phase (ME), Transition to Stationary (TS) and Late Stationary phase (LS). <br><br>Effect of FIS and H-NS on gene expression at relaxed and hypernegative supercoiling level was also studied using LZ41 and LZ54 strains. LZ41 and LZ54 strains contain drug-resistant alleles of different topoisomerase genes. In the LZ41 strain norfloxacin treatment strongly relaxes DNA, whereas in the LZ54 strain the same treatment generates high negative supercoiling (Khodursky et al., 1995, PNAS 92:11801-5; Ziechedrich et al, 1997, Genes Dev. 11:2580-92).

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