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Danio rerio

ABSTRACT: Estrogen sensing by GPER1 activates PI3K/mTOR to promote gender dimorphism in liver growth and cancer

PROVIDER: PRJNA358007 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5119940.fastq.gz	Fastqsanger.gz
	SRR5119941.fastq.gz	Fastqsanger.gz
	SRR5119942.fastq.gz	Fastqsanger.gz
	SRR5119943.fastq.gz	Fastqsanger.gz
	SRR5119944.fastq.gz	Fastqsanger.gz

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Estrogen sensing by GPER1 activates PI3K/mTOR to promote gender dimorphism in liver growth and cancer

Project description:Liver cancer is a common cause of cancer death, with a male-predominant incidence due, in part, to increased estrogen levels in cirrhotic patients. It is unknown, however, how estrogen is sensed to influence this process. Here, we show that estrogen activates the G protein-coupled estrogen receptor 1 (GPER1), expressed in hepatocytes, to enhancing hepatocyte size, cell cycle progression and cell proliferation, thereby increasing liver growth in zebrafish larvae and adults. GPER1 stimulation activates PI3K/mTOR signaling, and mTOR is essential for both normal and regenerative organ growth. Genetic loss of GPER1 diminishes and estrogen exposure accelerates chemical carcinogenesis, specifically in males. Chemical inhibition of GPER1 significantly reduces cancer incidence and progression in a gender-specific fashion. Our studies identify GPER1 as a hepatic estrogen sensor that mediates gender dimorphic growth, mTOR activation, and can serve as a therapeutic target for liver cancer treatment.

2019-05-29 | GSE92544 | GEO

Proteomic identification of the GPER1 interactome

Project description:The G-protein coupled estrogen receptor GPER1 is thought to mediate rapid estrogen signaling at the membrane. Despite considerable efforts over the last years, its physiological ligand(s), interactors, and regulators are still very poorly understood. This is particularly problematic at a time when the inducibility of GPER1 activity has been challenged. We therefore investigated both activity and interactome of GPER1. We could confirm that GPER1 can behave as a constitutivley active signaling protein. Using proximity labelling and immunoprecipitation coupled to mass spectrometry, we explored the GPER1 interactome. Intersecting the results of the two proteomic approaches and validating some candidate interactors, we generate a GPER1 a resource, which may be useful for further functional studies in the future.

2023-11-06 | PXD041943 | Pride

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Project description:Molecular basis of gender and reproductive status in breeding zebrafish

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Project description:Zebrafish: Estrogen induced gene expression

| PRJNA138129 | ENA

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Project description:Gender specific comparisons of gene expression during Zebrafish pectoral fin regeneration

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Identification of sexually dimorphic gene expression in the brain of adult zebrafish (Danio rerio)

Project description:Sexual differentiation in vertebrates is initiated during embryogenesis and leads to the development of individuals into phenotypic males or females. The molecular pathways showing sexual dimorphic patterns have been principally investigated in the gonads. In other organs, much less is known about the gender-associated pattern of gene expression. The brain plays a coordinating role on sexual function in both genders including regulation of reproductive development and maturation through the synthesis of neuropeptide hormones and the regulation of sexual behavior in both males and females. In this study, we aimed at identifying molecular pathways regulated in a gender specific manner in breeding zebrafish in order to generate a greater understanding of the gender specific physiology of the brain. To do so, we measured the gene expression profiles of individual brains of 6 males and 5 females using a 17k oligonucleotide microarray, and interrogated the dataset for genes showing a gender-specific gene expression profile. 42 genes were found to be differentially expressed between males and females from which 18 genes were over-expressed in males and 24 genes were over-expressed in females. Keywords: gender specific gene expression in the zebrafish brain

2008-01-01 | GSE6853 | GEO

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Project description:Sexual dimorphism in the zebrafish hepatic transcriptome and response to dietary carbohydrate

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Project description:Real-time quantitative PCR analysis of WT and NOD1 knockout zebrafish (mTor)

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Project description:Real-time quantitative PCR analysis of WT and NOD1 knockout zebrafish (PI3K-AKT)

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Project description:Dieldrin augments mTOR signaling and inhibits lysosomal acidification in the adult zebrafish heart (Danio rerio)