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Hyphosphere metagenome

ABSTRACT: Hyphae Raw sequence reads

PROVIDER: PRJNA358136 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR5121300.fastq.gz	Fastqsanger.gz
	SRR5121301.fastq.gz	Fastqsanger.gz
	SRR5121296.fastq.gz	Fastqsanger.gz
	SRR5121297.fastq.gz	Fastqsanger.gz
	SRR5121298.fastq.gz	Fastqsanger.gz

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Project description:Purpose: The goals of this study are to describe the morphological diversity of a novel archaea. Methods: The aerial and substrate hyphae of wild strain, and the substrate hyphae of mutated strains were generated by deep sequencing, in triplicate, using Illumina HiSeq2000 system. Results: Using an optimized data analysis workflow, we mapped about 1.7-4.4 million sequence reads per sample to wild and mutated hyphae samples by Bowti2 and RSeQC. Approximately half of the transcripts showed differential expression between the aerial and substrate hyphae, with a fold change ≥2.0 and p adjust <0.05. Conclusions: Our study represents the first detailed analysis of wild and mutated hyphae transcriptomes, with biologic replicates, generated by RNA-seq technology.

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