Project description:Dataset for a comparative analysis of protein profiles of three series of mouse macrophage cell line PMJ2R samples including infected with Tick-borne encephalitis virus, strain Hypr original isolate on the second (H2) and sixth day (H6) after infection was carried out using shotgun ultra-high resolution mass spectrometry.
Project description:Dataset for a comparative analysis of protein profiles of three series of mouse macrophage cell line PMJ2R samples including the control uninfected series (sample M) and infected with Tick-borne encephalitis virus, strain Hypr original isolate on the second (H2) and sixth day (H6) after infection was carried out using panoramic ultra-high resolution mass spectrometry. The analysis of measurements was performed using the proteomics search engine. The findings of the panoramic mass spectrometric analysis indicated that the protein composition of the cell samples is quite similar with respect to the number of identified proteins, protein composition and relative abundance. We identified a group of proteins specific for cell series H2 and H6.
Project description:Tick-borne encephalitis (TBE) is the most common tick-borne viral infection in Eurasia, with outcomes ranging from asymptomatic to fatal encephalitis. While the reasons for this variability are unclear, host genetics likely plays a role. Our previous research showed that BALB/c mice have intermediate susceptibility to TBE virus (TBEV), STS mice are highly resistant, and the recombinant congenic strain CcS-11, which has 12.5% of the STS genome on the BALB/c background, is more susceptible than the BALB/c. In this study, we used these three mouse strains to investigate the host response to TBEV infection in both peripheral macrophages, one of the initial target cell populations, and in the brain, the terminal target organ for the virus.
Project description:Upon tick borne encephalitis virus exposure of brain-resident cells, astrocytes are important IFN-β producers that followed a biphasic response, which initially depends on MAVS- and later on MyD88/TRIF-signaling