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Rattus norvegicus

ABSTRACT: RNA-Seq analysis of coding and long non-coding RNAs in the sub-chronic and chronic stages of spinal cord injury

PROVIDER: PRJNA360420 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5151130_1.fastq.gz	Fastqsanger.gz
	SRR5151130_2.fastq.gz	Fastqsanger.gz
	SRR5151131_1.fastq.gz	Fastqsanger.gz
	SRR5151131_2.fastq.gz	Fastqsanger.gz
	SRR5151132_1.fastq.gz	Fastqsanger.gz

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Project description:Chronic contusion spinal cord injury in rats

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Transcription profiling time series of Rattus norvegicus raphe magnus and sensorimotor cortex samples following moderate spinal cord injury

Project description:Summary: Spinal cord injury (SCI) is a damage to the spinal cord induced by trauma or disease resulting in a loss of mobility or feeling. SCI is characterized by a primary mechanical injury followed by a secondary injury in which several molecular events are altered in the spinal cord often resulting in loss of neuronal function. Analysis of the areas directly (spinal cord) and indirectly (raphe and sensorimotor cortex) affected by injury will help understanding mechanisms of SCI. Hypothesis: Areas of the brain primarily affected by spinal cord injury are the Raphe and the Sensorimotor cortex thus gene expression profiling these two areas might contribute understanding the mechanisms of spinal cord injury. Specific Aim: The project aims at finding significantly altered genes in the Raphe and Sensorimotor cortex following an induced moderate spinal cord injury in T9.

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Transcriptional study of the temporal evolution after severe traumatic spinal cord injury (SCI) and neural progenitor cells (NPCs)-transplantation intervention.

Project description:The present study is intended to disclose the transcriptional profile of the temporal evolution through the different stages after SCI as well as the molecular processes underlying the NPC based-therapy in order to transcriptionally characterize its therapeutical mechanism. This transcriptional profile analysis of total RNA samples from spinal cord homogenates of adult rats (Sprague Dawley) provides tissular (tought not cell-type specific) information of the critical time points after the injury from 1 to 8 weeks (acute, sub-acute and early-chronic and late-chronic stages) which conferred a wide temporal coverage making it ideal for studying the temporal dynamics of SCI. Furthermore, we have evaluated the impact of intramedullary acute or subacute transplantation of NPCs over the transcriptional regulation of the spinal cord tissue in order to define the functional outcomes of the NPCs therapy.

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