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Homo sapiens

ABSTRACT: Widespread Influence of 3′-end Structures on Mammalian mRNA Processing and Stability [CENPB-3'-end-library]

PROVIDER: PRJNA360952 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5167872.fastq.gz	Fastqsanger.gz
	SRR5167873.fastq.gz	Fastqsanger.gz
	SRR5167874.fastq.gz	Fastqsanger.gz
	SRR5167875.fastq.gz	Fastqsanger.gz
	SRR5167876.fastq.gz	Fastqsanger.gz

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Widespread Influence of 3′-end Structures on Mammalian mRNA Processing and Stability [Artificial-3'-end]

Project description:Understanding the physiological relevance of structures in mammalian mRNAs remains elusive, especially considering the global unfolding of mRNA structures in eukaryotic organisms recently examined, as well as the decade-long observation that mRNAs generally seem no more likely than random sequences to be stably folded. Here we show that RNA secondary structures, mostly weak and close-to-random, facilitate the 3′-end processing of thousands of human mRNAs by juxtaposing poly(A) signals (PASs) and cleavage sites that are otherwise too far apart. Folding of these 3′-end structures also enhances mRNA stability. Global structure probing shows that 3′-end regions are indeed folded in cells despite substantial unfolding of PAS-upstream regions. Analyses of thousands of ectopically expressed variants prove that folding both enhances processing and increases stability. Mutagenesis of a genomic locus further implicates structure-controlled processing in regulating neighboring gene expression. These results reveal widespread roles for RNA structure in mammalian mRNA biogenesis and metabolism.

2017-03-12 | GSE95467 | GEO