Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microplitis mediator

ABSTRACT: Microplitis mediator four tissues transcriptome

PROVIDER: PRJNA361063 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5177960_1.fastq.gz	Fastqsanger.gz
	SRR5177960_2.fastq.gz	Fastqsanger.gz
	SRR5177962_1.fastq.gz	Fastqsanger.gz
	SRR5177962_2.fastq.gz	Fastqsanger.gz
	SRR5177973_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Microplitis mediator

Project description:Microplitis mediator four tissues transcriptome

| PRJNA396461 | ENA

Microplitis mediator

Project description:Microplitis mediator overview

| PRJNA428779 | ENA

Microplitis mediator

Project description:Microplitis mediator Raw sequence reads

| PRJNA301549 | ENA

Microplitis mediator isolate:UGA

Project description:Microplitis mediator Genome

| PRJNA954320 | ENA

Microplitis mediator

Project description:The six developmental stages transcriptomes of Microplitis mediator

| PRJNA524252 | ENA

Microplitis mediator bracovirus

Project description:Microplitis mediator bracovirus Genome sequencing and assembly

| PRJNA319001 | ENA

Microplitis mediator isolate:Hebei

Project description:Microplitis mediator isolate:Hebei Genome sequencing and assembly

| PRJNA668670 | ENA

Genome sequencing of Microplitis mediator

Project description:Genome sequencing of Microplitis mediator adult male haploid

| PRJNA862959 | ENA

Microplitis mediator venom protein LC-MSMS

Project description:Insects mount an innate immune response to defend against the foreign invading microorganisms and parasites. To be successful parasites, endoparasitoid wasps need to be able to suppress the immune responses of their hosts. This allows the wasp eggs to hatch and the larvae to develop inside the host. However, the molecular mechanism underlying the interaction between wasp and its host remains largely unknown. In this study, we identified the reprolysin type metalloprotease venom component MmV189, which was designated venom regulatory factor-1 (VRF1). VRF1 plays a critical role in the interaction between the wasp Microplitis mediator and its lepidopteran host Helicoverpa armigera (the cotton bollworm). Proteomics analysis based on isobaric tags for relative and absolute quantitation (iTRAQ) revealed that at least twelve wasp venom proteins were released into the host hemolymph, causing significant changes in 511 host proteins at 24 h post parasitization. Taking an approach of integrated transcriptome and proteome analysis, we identified 313 putative proteins from the wasp venom reservoirs, 25 of which belong to the family of metalloproteases. Proenzyme of VRF1 was firstly cleaved in the host hemolymph after parasitization and then entered the hemocytes. Additionally, depletion of VRF1 in M. mediator by dsRNA-mediated knockdown significantly reduced the percentage of cocoon formation. Furthermore, we showed possible binding of VRF1 to Dorsal, a H. armigera nuclear factor kappa B (NF-κB), using yeast two-hybrid and pull-down assays, and confirmed the cleavage of Dorsal by VRF1. Moreover, we found that VRF1 acts as a protease to process Dorsal in the host hemocytes and suppress the induction of antimicrobial peptides (AMPs). Taken together, our findings identify a novel mechanism by which a component of endoparasitoid wasp venom interferes with host immune signaling cascades.

2019-01-07 | PXD007905 | Pride

Microplitis demolitor

Project description:Microplitis demolitor Parasitism Transcriptome

| PRJNA214515 | ENA