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Canis

ABSTRACT: Ancestry-specific methylation patterns in admixed offspring from an experimental coyote and gray wolf cross

PROVIDER: PRJNA361135 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5171230.fastq.gz	Fastqsanger.gz
	SRR5171240.fastq.gz	Fastqsanger.gz
	SRR5171231.fastq.gz	Fastqsanger.gz
	SRR5171232.fastq.gz	Fastqsanger.gz
	SRR5171233.fastq.gz	Fastqsanger.gz

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Project description:Background: The idea of using whole genome microarrays to detect peripheral blood biomarkers for physiological status a fairly new and unexplored method. Identifying biomarkers that can be linked to stress and immune response would be of great importance not only in animal management practices but in humans as well. The main objective of this research was to explore this concept using the North American Red Wolf (Canis rufus) which are exposed to a wide variety of environments from free ranging to confinement. Results: Transcription profiling of peripheral blood samples from 13 red wolf individuals in the Alligator River region of North Carolina revealed a strong signal of differentiation between confined and free-range animals. 482 out of 2,980 transcripts detected on the human Illumina Ref8 oligonucleotide bead arrays were found to differentiate these groups at a false discovery rate of 5 percent. Over-representation of genes in focal adhesion, insulin signaling, proteasomal, and tryptophan metabolism pathways suggests the activation of proinflammatory and stress responses in confined animals. Conclusions: Integration of immunological and physiological signals may leave a signature of lifestyle in the patterns of gene expression in the blood and suggest the possible development of biomarkers for disease and normal conditions. Keywords: Stress Response/Disease State

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