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5-methylcytosine promotes mRNA export

ABSTRACT: 5-methylcytosine promotes mRNA export

OTHER RELATED OMICS DATASETS IN: GSE93750GSE93749GSE93751

PROVIDER: PRJNA362296 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5183470_1.fastq.gz	Fastqsanger.gz
	SRR5183470_2.fastq.gz	Fastqsanger.gz
	SRR5183480_1.fastq.gz	Fastqsanger.gz
	SRR5183480_2.fastq.gz	Fastqsanger.gz
	SRR5183490_1.fastq.gz	Fastqsanger.gz

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Nuclear m6A Reader YTHDC1 Promotes Muscle Stem Cell Activation/Proliferation by Regulating mRNA Splicing and Nuclear Export [LACE-seq]

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Nuclear m6A Reader YTHDC1 Promotes Muscle Stem Cell Activation/Proliferation by Regulating mRNA Splicing and Nuclear Export [RNA-seq]

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Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP

Project description:The nuclear phase of the gene expression pathway culminates in the export of mature mRNAs to the cytoplasm through nuclear pore complexes (NPCs). GANP (Germinal-centre Associated Nuclear Protein) promotes the transfer to NPCs of mRNAs bound to the transport factor NXF1. Here, we demonstrate that GANP, subunit of the TREX-2 mRNA export complex, promotes selective nuclear export of a specific subset of mRNAs whose transport depends on NXF1. Genome-wide gene expression profiling showed that half of the transcripts whose nuclear export was impaired following NXF1 depletion also showed reduced export when GANP was depleted. GANP-dependent transcripts were highly expressed, yet short-lived, and were highly enriched in those encoding central components of the gene expression machinery such as RNA synthesis and processing factors. After injection into Xenopus oocyte nuclei, representative GANP-dependent transcripts showed faster nuclear export kinetics than representative transcripts that were not influenced by GANP depletion. We propose that GANP promotes the nuclear export of specific classes of mRNAs that may facilitate rapid changes in gene expression. We used gene expression profiling to compare the abundance of cytoplasmic RNAs after GANP or NXF1 depletion

2014-02-14 | E-GEOD-54481 | biostudies-arrayexpress

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Project description:The mRNA export receptor NXF1 coordinates transcriptional dynamics, alternative polyadenylation and mRNA export

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The Deubiquitylase Ubp15 Couples Transcription to mRNA Export

Project description:The nuclear export of messenger RNAs (mRNAs) is intimately coupled to their synthesis. pre-mRNAs assemble into dynamic ribonucleoparticles as they are being transcribed, processed and exported. The role of ubiquitylation in this process is increasingly recognized as the ubiquitylation of many key players have been shown to affect mRNA nuclear export. While a few E3 ligases have been shown to regulate nuclear export, evidence for deubiquitylases is currently lacking. Here, we identified the deubiquitylase Ubp15 as a regulator of nuclear export in Saccharomyces cerevisiae. Ubp15 interacts both with RNA polymerase II and with the nuclear pore complex, and its deletion reverts the nuclear export defect of mutants of the E3 ligase Rsp5. The deletion of UBP15 leads to hyper-ubiquitylation of the main nuclear export receptor Mex67 and affects its association with THO, a complex coupling transcription to mRNA processing and involved in the recruitment of mRNA export factors to nascent transcripts. Collectively, our data support a role for Ubp15 in coupling transcription to mRNA export.

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5-methylcytosine promotes mRNA export

Project description:This SuperSeries is composed of the SubSeries listed below.

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The mRNA export receptor NXF1 coordinates transcriptional dynamics, alternative polyadenylation and mRNA export

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Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP

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