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Homo sapiens

ABSTRACT: Genome-wide maps of chromatin state ATRA treated and control HL-60 cells [ChIP-seq]

PROVIDER: PRJNA363022 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5196910.fastq.gz	Fastqsanger.gz
	SRR5196911.fastq.gz	Fastqsanger.gz
	SRR5196912.fastq.gz	Fastqsanger.gz
	SRR5196905.fastq.gz	Fastqsanger.gz
	SRR5196906.fastq.gz	Fastqsanger.gz

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Human HL-60 Cells: all-trans retinoic acid (ATRA) vs. ATRA plus arsenic trioxide (ATO)

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Human HL-60 Cells: all-trans retinoic acid (ATRA) vs. ATRA plus arsenic trioxide (ATO)

Project description:Transcriptional profiling of human leukemia?HL-60 cells comparing ATRA treated HL-60 cells with ATRA plus ATO. Goal was to determine the effects of ATO on ATRA induced differentiation of HL-60 cells. Two-condition experiment, ATRA vs. ATRA plus ATO treated HL-60 cells.

2015-03-31 | E-GEOD-67420 | biostudies-arrayexpress

Genome-wide maps of chromatin state ATRA treated and control HL-60 cells [ChIP-seq]

Project description:These ChIP-seq data were generated to correlate with genomic interaction data in a related Hi-C analysis.The goal of ChIP-seq are to annotate active promoter and enhancer of ATRA treated and control HL-60 cells. After ATRA induction, H3K27ac peaks distal to gene promoters showed significant changes. Those changes were correlated with interaction intensity between promoters and enhancers.

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Differential Gene Expression between ATRA treated and control HL-60 Cells [RNA-seq]

Project description:These RNA-seq data were generated to correlate with genomic interaction data in a related Hi-C analysis. Analysis revealed differential expressed genes in ATRA treated and control HL-60 cells. Changes of gene expression with topological associated domains (TADs) were showd to be correlated with chromatin structure alteration

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Acute myeloid leukemia cell line RNA-Seq

Project description:We performed RNA-Seq on acute promyelocytic leukemia (APL) cells treated with both all-trans retinoic acid (ATRA) and trichostatin A (TSA) to identify transcriptional changes potentially associated with changes in APL cell mechanical properties. As a control, we performed the same treatments and RNA-Seq analysis on HL-60 cells, which were derived from a patient diagnosed with APL but whose cells lacked the hallmark fusion gene associated with APL. We performed RNA-Seq on these three cell lines (AP-1060, NB4, HL-60) under different conditions (untreated, TSA, ATRA, TSA + ATRA), and identified differentially expressed genes between samples and conditions. This study identified differentially expressed genes and enriched gene sets associated with (1) a resistance to differentiation induced by ATRA or (2) changes in mechanical properties. Overall, we find evidence that ATRA resistance and mechanical properties may be linked by the degree of chromatin condensation in APL cells.

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Project description:Differential Gene Expression between ATRA treated and control HL-60 Cells [RNA-seq]

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