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Sponge metagenome

ABSTRACT: Host-associated microbial community of the marine sponge Aplysina aerophoba from Gulf of Piran - sponge mesohyl, lysed by freeze-thaw cycling metagenome

PROVIDER: PRJNA366445 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8092709_1.fastq.gz	Fastqsanger.gz
	SRR8092709_2.fastq.gz	Fastqsanger.gz

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sponge metagenome

Project description:Host-associated microbial community of the marine sponge Aplysina aerophoba from Gulf of Piran - sponge pinacoderm, lysed by freeze-thaw cycling metagenome

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sponge metagenome

Project description:Host-associated microbial community of the marine sponge Aplysina aerophoba from Gulf of Piran - sponge mesohyl, lysed by bead beating metagenome

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sponge metagenome

Project description:Host-associated microbial community of the marine sponge Aplysina aerophoba from Gulf of Piran - sponge pinacoderm, lysed by bead beating metagenome

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sponge metagenome

Project description:Host-associated microbial community of the marine sponge Aplysina aerophoba from Gulf of Piran - sponge mesohyl, lysed by proteinase K digestion metagenome

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Aplysina aerophoba breed:marine sponge

Project description:Aplysina aerophoba breed:marine sponge Genome sequencing

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Transcriptomic profiles of the Mediterranean sponge species Aplysina aerophoba upon wounding by the specialist grazer Tylodina perversa

Project description:In the seabed, chemical defences mediate inter- and intraspecific interactions and may determine organisms’ success, shaping the diversity and function of benthic communities. Sponges represent a prominent example of chemically-defended marine organisms with great ecological success. The ecological factors controlling the production of their defensive compounds and the evolutionary forces that select for these defences remain little understood. Each sponge species produces a specific and diverse chemical arsenal with fish-deterrent, antifouling and antimicrobial properties. However, some small animals (mesograzers), mainly sea slugs, have specialized in living and feeding on sponges. Feeding on chemically-defended organisms provides a strategy to avoid predators, albeit the poor nutritional value of sponges. In order to investigate the mechanisms that control sponge chemical defence, with particular focus on the response to specialist grazers, we investigated the interaction between the sponge Aplysina aerophoba and the sea slug Tylodina perversa. Here we performed controlled experiments and collected sponge samples at different time points (3h, 1d and 6d after treatment). To further elucidate if the sponge response is specific to grazing by T. perversa, we also included a treatment in which sponges were mechanically damaged with a scalpel. We compared gene expression between treatments based on RNA-Seq data.

2022-01-30 | E-MTAB-8601 | biostudies-arrayexpress

Transcriptomic profiles of two sponge species upon exposure to microbial-associated molecular patterns (lipopolysaccharide and peptidoglycan) compared against control (sterile artificial seawater)

Project description:The interaction of animals with microbes relies on the specific recognition of microbial-derived molecules by receptors of the immune system. Sponges (phylum Porifera), as sister group of the Eumetazoa, provide insights into conserved mechanisms for animal-microbe crosstalk, but empirical data is limited. Here we aimed to characterize the immune response of sponges upon microbial stimuli by RNA-Seq. Two sponges species from the Mediterranean Sea, Aplysina aerophoba and Dysidea avara, were challenged with microbial-associated molecular patterns (lipopolysaccharide and peptidoglycan) or sterile artificial seawater (control) in aquarium experiments. Sponge tissue samples were collected 1h, 3h, and 5h after treatment. The response of the sponges to the treatments was assessed by differential gene expression analysis of RNA-Seq data. For each species, we compared the transcriptomic profiles of the samples in MAMP treatment to control within each time point.

2018-11-26 | E-MTAB-6757 | biostudies-arrayexpress

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Project description:Aplysina aerophoba isolate:Ligurian sea | breed:marine sponge Genome sequencing

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Transcription profiling of barley cv. Dicktoo response to low temperature stress

Project description:In this study we used the Affymetrix Barley 1 GeneChip to investigate transcriptome responses of barley cv. Dicktoo to low temperature, including triplicated measurements of cold, freeze/thaw cycles and de-acclimation over 33 days. Experiment Overall Design: Plants were grown at 20ÂºC for seven days and subject to a symmetrical cycle of acclimation, cold, freeze-thaw, and deacclimation. Chilling began by decreasing the temperature overnight from 20ÂºC to 4ÂºC at a rate of 1.3ÂºCâ?¢h-1 and maintaining temperatures of 4 ÂºC in the day and 2ÂºC at night for 5 days. Freeze-thaw cycling lasted 12 days with day temperatures of 4ÂºC and night temperatures gradually decreasing from -2ÂºC the first night to -4ÂºC for three nights and -10ÂºC for four nights, then recovering to -4ÂºC for three nights and -2ÂºC for one night. This treatment was designed to allow daily freeze-thaw cycling and protein synthesis. Chilling conditions (4ÂºC day, 2ÂºC night) were resumed for five days, followed by deacclimation with increasing temperature to 20ÂºC overnight and maintaining for three days. Sampling was done at four different times, each at the 11th hour of light to avoid circadian effects: 1) before chilling treatment, 2) five days after initiation of chilling treatment, 3) eight days into freeze-thaw treatment and 4) three days into de-acclimation.

2009-05-11 | E-GEOD-10329 | biostudies-arrayexpress

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