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Medicago truncatula

ABSTRACT: Ethylene signaling is important for isoflavonoid mediated resistance to Rhizoctonia solani in roots of Medicago truncatula

OTHER RELATED OMICS DATASETS IN: E-MTAB-5473

PROVIDER: PRJNA369210 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5217270_1.fastq.gz	Fastqsanger.gz
	SRR5217270_2.fastq.gz	Fastqsanger.gz
	SRR5217280_1.fastq.gz	Fastqsanger.gz
	SRR5217280_2.fastq.gz	Fastqsanger.gz
	SRR5217271_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Ethylene signaling is important for isoflavonoid mediated resistance to Rhizoctonia solani in roots of Medicago truncatula

Project description:The root-infecting necrotrophic fungal pathogen Rhizoctonia solani causes significant disease to all the world’s major food crops. As a model for pathogenesis of legumes we have examined the interaction of R. solani AG8 with Medicago truncatula. RNAseq analysis of the moderately resistant M. truncatula accession A17 and highly susceptible sickle (skl) mutant (defective in ethylene sensing) identified major transcriptional reprogramming early in A17. Responses specific to A17 included components of ethylene signalling, numerous class IX ERF transcription factor family members, reactive oxygen species metabolism and consistent up-regulation of the isoflavonoid biosynthesis pathway. Mass-spectrometry revealed accumulation of the isoflavonoid related compounds liquiritigenin, formononetin, medicarpin and biochanin A in A17. Over-expression of an isoflavone synthase (IFS) in M. truncatula roots increased isoflavonoid accumulation and resistance to R. solani. Addition of exogenous medicarpin suggested this phytoalexin may be one of several isoflavonoids required to contribute to resistance to R. solani. Together these results provide evidence for the role of ethylene-mediated accumulation of isoflavonoids during defence against root pathogens in legumes.

2017-06-06 | GSE94260 | GEO

RNA-seq of Medicago truncatula A17 and the ethylene insensitive sickle mutant interacting with the fungal root pathogen, Rhizoctonia solani AG8

Project description:This study compares the response of wild type (A17) and ethylene insensitive mutant (sickle) lines of the model legume Medicago truncatula to infection by the root-infecting necrotrophic fungal pathogen, Rhizoctonia solani AG8 (WAC10335). Two time points were taken, 2 and 7 days after inoculation along with corresponding mock-treated controls.

2017-06-06 | E-MTAB-5473 | biostudies-arrayexpress

Transcriptom analysis of the interaction Medicago truncatula – Ralstonia solanacearum

Project description:affy_ralstonia_medicago - Ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease. Its infection process was studied with an in vitro inoculation procedure on intact roots of Medicago truncatula. The pathosystem involved susceptible A17 and resistant F83005.5 M truncatula lines infected with the pathogenic strain GMI1000. The mutant A17 line, Sickle, which showed a resistant phenotype was also part of the experiment. To identify host signaling pathway triggered by R. solanacearum infection with a focus on the involvment of ethylene, we used the Medicago Affymetrix array to monitore the expression profiles and the molecular process associated with initial symptoms development (12hpi) and colonization (72hpi). In order to maximize chances to observe differential gene expression, RNA samples were extracted from the root infection zone (root tips) -Three Medicago truncatula lines, A17, F83005.5 and sickle were inoculated with GMI1000 Ralstonai solanacearum strain (107 cfu/ml). RNA were extracted from root extremities (1 cm above the root tip) at time 0, 12h and 72h post inoculation. Three biological repeats were conducted

2011-09-10 | GSE18473 | GEO

Transcriptom analysis of the interaction Medicago truncatula – Ralstonia solanacearum

2011-09-09 | E-GEOD-18473 | biostudies-arrayexpress

Effects of Gongronella sp. w5 on Medicago truncatula transcriptome

Project description:To investigate the gene expression levels of Medicago truncatula roots after beneficial fungi Gongronella sp. w5 inoculated.Gongronella sp. w5 promoted M. truncatula growth and caused the accumulation of sucrose in M. truncatula root tissue at 16 day-post-inoculation (dpi) without invading into the root cells. The transport of photosynthetic product sucrose to the rhizosphere by M. truncatula root cells was accelerated by upregulating the SWEET gene.

2020-08-11 | GSE155951 | GEO

Comparative proteomic analysis reveals differential root proteins in Medicago sativa and Medicago truncatula in response to salt stress

Project description:we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively.

2016-04-14 | PXD003761 | Pride

Overexpression of MtABI3 in transgenic Medicago truncatula roots

Project description:ABI3 is a B3-domain transcription factor that acts as a master regulator of seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Medicago hairy roots were generated using Agrobacterium rhizogenes transformed with the genomic sequence of the ABI3 gene of Medicago. Using the Medicago NimbleGen chip, a transciptomic analysis was performed to identify differentially expressed genes compared to the GUS expressed control.

2013-02-14 | GSE44291 | GEO

Medicago truncatula seedlings grown two weeks in hydroponics media then carry out salt stress

Project description:time-course salt stress experiment of model legume Medicago truncatula roots using Affymetrix Medicago Array, aimed to dig some useful gene for improve salt resistance for legumes and other crops

2008-12-12 | GSE13907 | GEO

Transcript analysis of early nodulation events in Medicago truncatula

Project description:For transcript analysis of early nodulation events in Medicago truncatula we compared transcripts from inoculated and uninoculated roots corresponding to defined stages between 1 and 72 h post inoculation (hpi). Keywords: time course

2005-10-14 | GSE3441 | GEO

Expression data of Medicago truncatula Jemalong A17 roots treated with auxin transport inhibitors

Project description:Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. The transcriptional response of M. truncatula roots 1 and 7 days after ATI treatment were opposite to roots treated with S. meliloti.

2011-03-26 | GSE27991 | GEO

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