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Homo sapiens

ABSTRACT: Transcriptome characterization of human highly osteoblastic/cementoblastic periodontal ligament cell clones (OM medium)

PROVIDER: PRJNA369316 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5222820_1.fastq.gz	Fastqsanger.gz
	SRR5222820_2.fastq.gz	Fastqsanger.gz
	SRR5222821_1.fastq.gz	Fastqsanger.gz
	SRR5222821_2.fastq.gz	Fastqsanger.gz
	SRR5222822_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Homo sapiens

Project description:Transcriptome characterization of human periodontal ligament cell clones (DMEM medium)

| PRJNA369314 | ENA

Effects of hair keratin on cell cycle regulation of human periodontal ligament cells in histidine-tryptophan-ketoglutarate-based storage media

Project description:In this study, the effect of a storage medium (hK-HTCM) in which hair keratin was dissolved in a 1:1 mixed solution of Histidine-Tryptophan-Ketoglutarate and Culture media solution (HTCM) was evaluated on the viability and proliferation of human periodontal ligament cells. There was no difference in cytotoxicity between 0.1% and 0.25% hK-HTCM against 0% hK-HTCM and human periodontal ligament cells. Human periodontal ligament cells were cultured in 0.1% and 0.25% hK-HTCM for 48 hours, and after removing hair keratin from the culture medium, the cells resumed proliferation. When exposed to 0.25% hK-HTCM, human periodontal ligament cells showed differential expression of genes related to cell cycle and cell division regulation. On the other hand, differential expression of genes related to phosphorylation and ubiquitination related to cell cycle resumption was observed in human periodontal ligament cells after removal of 0.25% hK-HTCM. 0.25% hK-HTCM showed the ability to regulate the cell cycle of human periodontal ligament cells without showing cytotoxicity, and its potential to be used as a long-term storage medium for avulsed teeth was confirmed.

2024-06-15 | GSE269474 | GEO

Reverse Transcription quantitative PCR (RT-q PCR) analysis of osteogenic-related markers for osteogenic differentiation from human periodontal ligamen stem cells

Project description:We pre-treated human periodontal ligament stem cells (hPDLSCs) under Iinterleukin-6 (IL-6) in osteogenic inductive medium (OM) on osteogenic differentiation potential and investigate the specific signaling pathway by clarifying with several antagonists (Dkk-1, SP600125, SB431542, and DAPT) and siRNA (siWnt2B, siWnt10B, and siWnt5A) to identify the differences in the expression pattern of osteogenic related markers by reverse transcription quantitative polymerase chain reaction (RT-qPCR).

2023-02-19 | GSE225547 | GEO

Expression data from human periodontal ligament

Project description:We used microarrays to detect the differences in gene-expression of the periontal ligament between patients with healthy periodontal ligament and patients with periodontitis RNA was extracted directly from the middle third of the human periodontal ligament

2011-03-17 | E-GEOD-27993 | biostudies-arrayexpress

Maresin-1 and Resolvin E1 promote regenerative properties of periodontal ligament stem cells under inflammatory conditions

Project description:Maresin-1 (MaR1) and Resolvin E1 (RvE1) are specialized pro-resolving lipid mediators (SPMs) that regulate inflammatory processes. We have previously demonstrated the hard and soft tissue regenerative capacity of RvE1 in an in vivo model of periodontal disease characterized by inflammatory tissue destruction. Regeneration of periodontal tissues requires a well-orchestrated processes mediated by periodontal ligament stem-cells. However, limited data are available on how SPMs can regulate the regenerative properties of human periodontal ligament stem cells (hPDLSCs) under inflammatory conditions. Thus, we measured the impact of MaR1 and RvE1 in an in vitro model of hPDLSCs after stimulation with IL-1β and TNF-α by evaluating pluripotency, migration, proliferation, cell death, periodontal ligament markers (α-smooth muscle actin, tenomodulin, and periostin), cementum-osteogenic differentiation and phosphoproteomic perturbations. The data showed that the inflammatory milieu suppresses pluripotency, proliferation and migration of hPDLSCs; MaR1 and RvE1 both restored regenerative capacity by increasing hPDLSC proliferation, accelerating wound healing/migration, and upregulating periodontal ligament markers and cementum-osteogenic differentiation. Protein phosphorylation perturbations were associated with the SPM-induced regenerative capacity of hPDLSCs. Together, these results demonstrate that MaR1 and RvE1 restore or improve the regenerative properties of highly specialized stem cells when inflammation is present and offer opportunities for direct pharmacologic treatment of lost tissue integrity.

2021-09-09 | PXD019546 | Pride

Recombinant irisin enhances the differentiation, remodeling potential, and the mechanical properties of 3D-cultured human periodontal ligament cells

Project description:Irisin is recognized as a myokine produced by muscles, regulating metabolism and energy homeostasis, however, it may play a role in many other biological functions. Little is known about its effect on periodontal ligament cells. We employed Affymetrix to profile mRNA expression patterns between 3D human periodontal ligament cell spheroids treated with and without irisin. The mRNA expression profiling identified approximately 1000 mRNAs to be differentially expressed between the two groups, which suggests that irisin is involved in gene regulation in human periodontal ligament cells.

2022-06-25 | GSE206938 | GEO

Differentially expressed long noncoding RNA expression between periodontal ligament stem cells from healthy periodontal tissue and periodontal ligament stem cells from inflammatory periodontal tissue.

Project description:Periodontitis can impair the osteogenic differentiation of human periodontal mesenchymal stem cells, but the underlying molecular mechanisms are still poorly understood. Long noncoding RNAs (lncRNAs) have been demonstrated to play significant roles under both physiologic and pathological conditions. We performed comprehensive lncRNAs profiling by lncRNA microarray to identify differentially expressed long noncoding RNA expression between Periodontal ligament stem cells from healthy Periodontal tissue and periodontal ligament stem cells from inflammatory periodontal tissue. Our analysis identified 233 lncRNAs and 423 mRNAs that were differently expressed (fold change >2.0, p-value < 0.05) between the two groups of cells. The GO analysis revealed that the significantly down-regulated biological processes included multicellular organismal process, developmental process and multicellular organismal development and the significantly up-regulated biological processes included cellular process, biological regulation and response to stimulus in periodontal ligament stem cells from inflammatory periodontal tissue. The Pathway analysis revealed that the differentially expressed mRNAs may involved in Focal adhesion, ECM-receptor interaction, Bacterial invasion of epithelial cells, Long-term depression, Circadian entrainment and HIF-1 signaling pathway. Two-condition experiment, periodontal ligament stem cells from healthy periodontal tissue (hPDLSCs) vs. periodontal ligament stem cells from inflammatory periodontal tissue (pPDLSCs), Biological replicates: 3 control replicates (hPDLSCs), 3 testing replicates (pPDLSCs).

2016-02-19 | E-GEOD-78074 | biostudies-arrayexpress

Gene expression analysis of human permanent periodontal ligament tissue

Project description:Gne expression analysis data of human permanent periodontal ligament tissues

2018-12-31 | GSE75695 | GEO

Effect of FDC-SP on Gene Expression of Human Periodontal Ligament Cells

Project description:Previous research has reported that FDC-SP had similar molecular properties to statherin, a protein exists in saliva which plays important roles in preventing Ca precipitation. Further biomolecular study has suggested that the expression of FDC-SP may be associated with periodontal ligament (PDL) phenotype expression. Therefore, we hypothesized that FDC-SP may play specific roles in the inhibition of calcium precipitation during periodontal regeneration, as well as affect phenotype expression of periodontal ligament cells (PDLCs) during the differentiation process. To investigate this, we applied microarray technology to identify gene expression changes in hPDLCs transfected with FDC-SP and then clustered them according to their biological functions. We firstly established a recombinant lentiviral vector containing FDC-SP and obtained safe and efficient FDC-SP overexpression in human periodontal ligament cells (hPDLCs). After that, we applied Agilent Whole Human Genome Oligo Microarray (4M-CM-^W44K) to identify differentially expressed genes between empty vector-transfected hPDLCs and FDC-SP -transfected ones and then clustered them according to their biological functions. 3 independent experiments were performed and the empty vector-transfected hPDLCs were used as control.

2014-09-13 | E-GEOD-61384 | biostudies-arrayexpress

Comparative gene expression analysis of the human periodontal ligament and dental pulp in the human permanent teeth

Project description:The periodontal ligament(PDL) and dental pulp tissues of human permanent teeth have a number of differences in their developmental processes, histological characteristics and functions. It can be figured out that these differences are attributable to genetic backgrounds of their cells organized tissues. The purpose of this study was to identify the gene-expression profiles and their molecular biological differences of periodontal ligament and dental pulp tissues from the human permanent teeth.

2016-11-22 | GSE50639 | GEO

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