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Prunus avium

ABSTRACT: A hydrogen cyanamide-responsive transcriptome of sweet cherry flower buds from endodormancy to flowering

PROVIDER: PRJNA369332 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5219260_1.fastq.gz	Fastqsanger.gz
	SRR5219260_2.fastq.gz	Fastqsanger.gz
	SRR5219270_1.fastq.gz	Fastqsanger.gz
	SRR5219270_2.fastq.gz	Fastqsanger.gz
	SRR5219261_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

A hydrogen cyanamide-responsive transcriptome of sweet cherry flower buds from endodormancy to flowering

Project description:Perennial plants, like fruit trees grown in temperate regions, are characterized by bud dormancy, a rest state that protects the bud from cold during winter. At the same time, these plants have developed a requirement for winter chill for correct flowering. However, winters are becoming increasingly warm in temperate regions, resulting in dramatic effects on the flowering output and therefore crop yield. A compound that successfully compensates for missing winter chill is hydrogen cyanamide, which has been used to synchronize and advance flowering time in a range of commercially important fruit crops. Hydrogen cyanamide also represents a unique tool for researchers to study controlled endodormancy release. Here, we treated dormant sweet cherry flower buds with hydrogen cyanamide, sampling flower buds at different time points after treatment. RNAseq revealed more than 6,000 hydrogen cyanamide-responsive genes. In accordance with these results, hydrogen cyanamide treatment increased the levels of jasmonoyl-isoleucine (JA-Ile) and the cytokinins trans-zeatin riboside (tZR), dihydrozeatin (DZ) and dihydrozeatin riboside (DZR). Furthermore, hydrogen cyanamide affected the expression of antioxidant- and cell wall loosening-associated transcripts. These results suggest a complex mechanism of action for hydrogen cyanamide-induced endodormancy release, including key roles for JA-Ile, zeatin-type cytokinins and hydrogen cyanide.

2017-06-01 | GSE94290 | GEO

Trancriptomic analysis of flower bud dormancy in sweet cherry

Project description:Bud dormancy is a crucial stage in perennial trees and allows survival over winter and optimal subsequent flowering and fruit production. Environmental conditions, and in particular temperature, have been shown to influence bud dormancy. Recent work highlighted some physiological and molecular events happening during bud dormancy in trees. However, we still lack a global understanding of transcriptional changes happening during bud dormancy. We conducted a fine tune temporal transcriptomic analysis of sweet cherry (Prunus avium L.) flower buds from bud organogenesis until the end of bud dormancy using next-generation sequencing. We observe that buds in organogenesis, paradormancy, endodormancy and ecodormancy are characterised by distinct transcriptional states, and associated with different pathways. We further identified that endodormancy can be separated in two phases based on its transcriptomic state: early and late endodormancy. We also found that transcriptional profiles of just 7 genes are enough to predict the main cherry tree flower buds dormancy stages. Our results indicate that transcriptional changes happening during dormancy are robust and conserved between different sweet cherry cultivars. Our work also sets the stage for the development of a fast and cost effective diagnostic tool to molecularly define the flower bud stage in cherry trees.

2019-12-16 | GSE130426 | GEO

Transcriptomic analysis of flower bud dormancy in the sweet cherry cultivar 'Fertard'

Project description:Temperate perennial plants have to adapt their growth in order to survive over winter conditions and protect their tissues by entering into dormancy. The dormancy phenomenon is a complex process regulated by many factors, externals (photoperiod and temperature) or internals through the transport towards the buds. In order to better understand the molecular mechanisms involved in the regulation of dormancy, we conducted a transcriptomic analysis of flower buds from the late flowering sweet cherry cultivar 'Fertard' during dormancy, from October 2017 to March 2018.

2023-04-15 | GSE229429 | GEO

Project description:sweet cherry flower buds dormancy

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Project description:sweet cherry flower buds dormancy

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Prunus avium transcriptome profiling

Project description:Profiling of fruit and leaves transcriptomes of cherry cultivars

2019-02-26 | GSE121997 | GEO

Prunus avium

Project description:Identification of self-incompatibility modifier in sweet cherry

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Project description:Transcriptome sequencing of sweet cherry flower buds

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Prunus avium cultivar:Tieton

Project description:Sweet cherry (Prunus avium) cv. Tieton Raw sequencing data

| PRJNA503752 | ENA

Hydrogen cyanamide breaks grapevine bud dormancy in the summer

Project description:Microarray analysis revealed specific alterations in gene expression in dormancy breaking buds induced by pruning (P), hydrogen cyanamide (HC), pruning plus hydrogen cyanamide (PHC) after 24 h of treatment. PHC treatment altered the expression of the largest number of genes and rapid accumulation of a sublethal level of reactive oxygen species and reactive nitrogen species subsequently induces cell wall loosening and expansion for bud sprouting

2016-08-30 | GSE86163 | GEO

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