Project description:Background: Pantoea ananatis LMG 2665T synthesizes and utilizes acyl homoserine lactones (AHLs) for signaling. In this strain, short chain AHLs (C4 to C8) are produced by the EanI/R quorum sensing (QS) system that is involved in pathogenicity and biofilm formation. The complete set of genes regulated by the EanI/R system in P. ananatis LMG 2665T is still not fully known. In the present study, RNA-seq was used to analyze the transcriptome profiles controlled by the EanI/R system in this strain by comparing the wild type strain and its QS mutant 2665T ean∆I/R during lag and log stages. The RNA seq data was validated by RT qPCR. Results: The results showed that the EanI/R regulon in P. ananatis LMG 2665T comprised 144 genes, constituting 3.3% of the whole transcriptome under the experimental conditions in this study. The majority of genes regulated by the EanI/R system included genes for flagella assembly, bacterial chemotaxis, pyruvate metabolism, two component system, metabolic pathways, microbial metabolism and biosynthesis of secondary metabolites. Conclusions: This is the first study to identify the EanI/R QS regulon in P. ananatis LMG 2665T. Functional analysis of genes regulated the EanI/R system in LMG 2665T could help unveil genes that play a vital role in pathogenesis and survival strategies of this pathogen.

2018-10-31 | GSE87207 | GEO

Noviherbaspirillum canariense strain:CECT 7838 | isolate:CECT 7838

Project description:Noviherbaspirillum canariensis CECT 7838 genome sequencing

| PRJNA459746 | ENA

Noviherbaspirillum malthae strain:JCM 18414 | isolate:JCM 18414

Project description:Noviherbaspirillum malthae JCM 18414 genome sequencing

| PRJNA364431 | ENA

Noviherbaspirillum suwonense strain:DSM 26001 | isolate:DSM 26001

Project description:Noviherbaspirillum suwonense DSM 26001 genome sequencing

| PRJNA369944 | ENA

Noviherbaspirillum malthae strain:JCM 18414 | isolate:JCM 18414

Project description:Noviherbaspirillum malthae JCM 18414 genome sequencing