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Rexinoid signalling during early myogenic differentiation

ABSTRACT: Rexinoid signalling during early myogenic differentiation

PROVIDER: PRJNA371483 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5233110.fastq.gz	Fastqsanger.gz
	SRR5233120.fastq.gz	Fastqsanger.gz
	SRR5233111.fastq.gz	Fastqsanger.gz
	SRR5233121.fastq.gz	Fastqsanger.gz
	SRR5233102.fastq.gz	Fastqsanger.gz

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RNA-seq profiling of rexinoid responsive gene expression during early myogenic differentiation

Project description:While skeletal myogenesis is tightly coordinated by myogenic regulatory factors including MyoD and myogenin, chromatin modifications have emerged as vital mechanisms of myogenic regulation. We have previously established that bexarotene, a clinically approved agonist of retinoid X receptor, promotes the specification and differentiation of skeletal muscle lineage. Here, we examine a genome-wide impact of rexinoids on myogenic differentiation through integral RNA-seq and ChIP-seq analyses. We found that bexarotene promotes myoblast differentiation through the coordination of exit from the cell cycle and the activation of muscle-related genes. We uncovered a new mechanism of rexinoid action which is mediated by the nuclear receptor and largely reconciled through a direct regulation of MyoD gene expression. In addition, we determined a rexinoid-responsive residue-specific histone acetylation at a distinct chromatin state associated to MyoD and myogenin. Thus, we provide novel molecular insights into the interplay between retinoid X receptor signaling and chromatin states pertinent to myogenic programs in early myoblast differentiation.

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ChIP-seq profiling of myogenin and p300 occupancy in the context of rexinoid signaling during early myogenic differentiation

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