Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Halopseudomonas pachastrellae

ABSTRACT: Pseudomonas pachastrellae overview

PROVIDER: PRJNA373726 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR4235007_1.fastq.gz	Fastqsanger.gz
	SRR4235007_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Halopseudomonas pelagia

Project description:Pseudomonas pelagia overview

| PRJNA198442 | ENA

Halopseudomonas aestusnigri

Project description:Pseudomonas aestusnigri overview

| PRJNA391364 | ENA

Halopseudomonas bauzanensis

Project description:Pseudomonas bauzanensis overview

| PRJNA303902 | ENA

Halopseudomonas oceani

Project description:Halopseudomonas oceani overview

| PRJNA1043283 | ENA

Halopseudomonas species: Cultivation and molecular genetic tools.

Project description:The Halopseudomonas species, formerly classified as Pseudomonas pertucinogena lineage, form a unique phylogenetic branch within the Pseudomonads. Most strains have recently been isolated from challenging habitats including oil- or metal-polluted sites, deep sea, and intertidal zones, suggesting innate resilience to physical and chemical stresses. Despite their comparably small genomes, these bacteria synthesise several biomolecules with biotechnological potential and a role in the degradation of anthropogenic pollutants has been suggested for some Halopseudomonads. Until now, these bacteria are not readily amenable to existing cultivation and cloning methods. We addressed these limitations by selecting four Halopseudomonas strains of particular interest, namely H. aestusnigri, H. bauzanensis, H. litoralis, and H. oceani to establish microbiological and molecular genetic methods. We found that C4 -C10 dicarboxylic acids serve as viable carbon sources in both complex and mineral salt cultivation media. We also developed plasmid DNA transfer protocols and assessed vectors with different origins of replication and promoters inducible with isopropyl-β-d-thiogalactopyranoside, l-arabinose, and salicylate. Furthermore, we have demonstrated the simultaneous genomic integration of expression cassettes into one and two attTn7 integration sites. Our results provide a valuable toolbox for constructing robust chassis strains and highlight the biotechnological potential of Halopseudomonas strains.

| S-EPMC10832565 | biostudies-literature

Halopseudomonas bauzanensis strain:cgmcc 1.9095

Project description:Pseudomonas bauzanensis CGMCC 1.9095 genome sequencing

| PRJNA303332 | ENA

Halopseudomonas litoralis strain:2SM5 | isolate:KCTC 23093

Project description:Pseudomonas litoralis CECT 7670 genome sequencing

| PRJNA323019 | ENA

Halopseudomonas aestusnigri strain:CECT 8317 | isolate:CECT 8317

Project description:Pseudomonas aestusnigri VGX014 genome sequencing

| PRJNA319693 | ENA

Halopseudomonas bauzanensis strain:DSM 22558 | isolate:DSM 22558

Project description:Pseudomonas bauzanensis LMG 26048 genome sequencing