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Gossypium

ABSTRACT: Gossypium arboreum,Gossypium raimondii，Gossypium hirsutum and Gossypium arboreum x Gossypium raimondii F1 Raw sequence reads

PROVIDER: PRJNA373801 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR5468500_1.fastq.gz	Fastqsanger.gz
	SRR5468500_2.fastq.gz	Fastqsanger.gz
	SRR5468510_1.fastq.gz	Fastqsanger.gz
	SRR5468510_2.fastq.gz	Fastqsanger.gz
	SRR5468501_1.fastq.gz	Fastqsanger.gz

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Gossypium

Project description:ssRNA-seq sequencing of Gossypium arboreum, Gossypium raimondii， Gossypium hirsutum，Gossypium arboreum x Gossypium raimondii F1 Raw sequence reads

| PRJNA371400 | ENA

Transcriptomics and Metabolomics of 10 - 28 day post anthesis cotton fiber from Gossypium hirsutum and Gossypium barbadense

Project description:Purpose: The goal of this experiment was to use RNA-seq to compare the two commercial cotton species Gossypium hirsutum and Gossypium barbadense and determine what transcripts may account for the better fiber quality in the latter. Methods: RNA was extracted from Gossypium barbadense or Gossypium hirsutum fibers at 10, 15, 18, 21, and 28 days post anthesis. Paired-end, 100-bp RNA-seq was performed on an Illumina HiSeq2000 and the reads were mapped to the Gossypium raimondii genome at www.phytozome.net and non-homologous contig assemblies from Gossypium arboreum. Results from RNA-seq were combined with non-targeted metabolomics. Results: Approximately 38,000 transcripts were expressed (RPKM>2) in each fiber type and approximately 2,000 of these transcripts were differentially expressed in a cross-species comparison at each timepoint. Enriched Gene Ontology biological processes in differentially expressed transcripts suggested that Gh fibers were more stressed. Conclusions: Both metabolomic and transcriptomic data suggest that better mechanisms for managing reactive oxygen species contribute to the increased fiber length in Gossypium barbadense. This appears to result from enhanced ascorbate biosynthesis via gulono-1,4-lactone oxidase and ascorbate recycling via dehydroascorbate reductase. See Bioproject PRJNA263926 and SRA accession SRP049330 for study design and raw sequencing data and Bioproject PRJNA269608 and TSA accession GBYK00000000 for Gossypium arboreum assembled contig sequences used for transcriptome mapping - Cotton fiber mRNA from 10,15,18,21 and 28 day post anthesis fiber from either Gossypium hirusutm or Gossypium barbadense was sequenced and differential gene expression analysis was conducted between species for each timepoint and between adjacent timepoints. Each timepoint was representative of fiber from 9 individual plants processed as 3 biological replicate pools (material from 3 individual plants per pool).

2015-06-26 | E-GEOD-64958 | biostudies-arrayexpress

Transcriptomics and Metabolomics of 10 - 28 day post anthesis cotton fiber from Gossypium hirsutum and Gossypium barbadense

2015-06-26 | GSE64958 | GEO

Gossypium

Project description:Small RNA sequencing of Gossypium arboreum x Gossypium raimondii F1

| PRJNA375828 | ENA

Drougt Specfic oligonucleotide array Development for drought response study in Gossypium Species

Project description:Drought is one of the primary limiting factors affecting the growth and yield of cotton. Studying the genotypic drought response of plant towards stress stimuli necessitates the development of a standardized, comprehensive and cohesive system that specifically captures information regarding the focal purpose. In-house development of drought specific microarray using drought specific oligonucleotide probes was carried out and the leaf and root tissue of the two-important species Gossypium arboreum and Gossypium hirsutum were tested for genetic traits responses under 10 days’ drought stress. Further the response of these tissue under control and drought stress were studied via inhouse developed oligonucleotide chip.

2021-06-03 | GSE116908 | GEO

Gossypium arboreum

Project description:Gossypium arboreum Raw sequence reads

| PRJNA712876 | ENA

Gossypium arboreum

Project description:Gossypium arboreum raw sequence reads

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Gossypium arboreum

Project description:Gossypium arboreum Raw sequence reads

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Gossypium arboreum

Project description:Gossypium arboreum Raw sequence reads