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Krox20 hindbrain regulation involves multiple modes of cooperation between cis-acting elements

ABSTRACT: Krox20 hindbrain regulation involves multiple modes of cooperation between cis-acting elements

PROVIDER: PRJNA373839 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5219560_1.fastq.gz	Fastqsanger.gz
	SRR5219560_2.fastq.gz	Fastqsanger.gz
	SRR5219570_1.fastq.gz	Fastqsanger.gz
	SRR5219570_2.fastq.gz	Fastqsanger.gz
	SRR5219580_1.fastq.gz	Fastqsanger.gz

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Krox20 hindbrain regulation involves multiple modes of cooperation between cis-acting elements [ATAC-seq]

Project description:Many genes harbour multiple transcriptional enhancers that act concomitantly to achieve robust and precise spatial-temporal expression. In vertebrates, however, the mechanisms underlying cooperation between cis-acting elements are poorly documented. The mouse gene Krox20 encodes a transcription factor required for the specification of two segments (rhombomeres) of the developing hindbrain. In rhombomere 3, Krox20 is subject to positive feedback, governed by enhancer A, which is directly bound by the KROX20 protein, whereas another element, C, distant from 70 kb, was supposed to be only required for initiation of expression. Here, using both enhancer knock-outs and investigations of chromatin organisation, we show that element C possesses a dual activity: besides its classical enhancer function, it is also permanently required in cis to potentiate element A autoregulatory activity, by increasing its chromatin accessibility. This work uncovers a novel, asymmetrical, long-range mode of cooperation between cis-acting elements that might be essential to avoid promiscuous activation of positive autoregulatory elements.

2017-07-18 | GSE97102 | GEO

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Project description:Krox20 hindbrain regulation involves multiple modes of cooperation between cis-acting elements [4C-seq]

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Mus musculus

Project description:Krox20 hindbrain regulation involves multiple modes of cooperation between cis-acting elements [ChIP-Seq]

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Mus musculus

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Krox20 hindbrain regulation involves multiple modes of cooperation between cis-acting elements

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-07-18 | GSE94716 | GEO

Krox20 hindbrain regulation incorporates multiple modes of cooperation between cis-acting elements.

Project description:Developmental genes can harbour multiple transcriptional enhancers that act simultaneously or in succession to achieve robust and precise spatiotemporal expression. However, the mechanisms underlying cooperation between cis-acting elements are poorly documented, notably in vertebrates. The mouse gene Krox20 encodes a transcription factor required for the specification of two segments (rhombomeres) of the developing hindbrain. In rhombomere 3, Krox20 is subject to direct positive feedback governed by an autoregulatory enhancer, element A. In contrast, a second enhancer, element C, distant by 70 kb, is active from the initiation of transcription independent of the presence of the KROX20 protein. Here, using both enhancer knock-outs and investigations of chromatin organisation, we show that element C possesses a dual activity: besides its classical enhancer function, it is also permanently required in cis to potentiate the autoregulatory activity of element A, by increasing its chromatin accessibility. This work uncovers a novel, asymmetrical, long-range mode of cooperation between cis-acting elements that might be essential to avoid promiscuous activation of positive autoregulatory elements.

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Krox20 hindbrain regulation in Zebrafish [ATAC-seq]

Project description:Krox20 hindbrain regulation in Zebrafish [ATAC-seq]

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Krox20 hindbrain regulation in Zebrafish [ATAC-seq]

Project description:In the present study, we have identified six enhancers located within the zebrafish krox20 locus, using accessibility data generated by ATAC-seq and characterized their activity profiles as well as their functions and interactions in the zebrafish hindbrain . We also investigated the orthologues in six evolutionary distant vertebrates of one of these element, showing a great variety of activity profiles demonstrating the striking evolutionary flexibility of this element .

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