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Sulfolobus acidocaldarius

ABSTRACT: Identification of the Sulfolobus acidocaldarius small RNome and L7Ae-RNA interactome

PROVIDER: PRJNA373915 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR5241990.fastq.gz	Fastqsanger.gz
	SRR5241991.fastq.gz	Fastqsanger.gz
	SRR5241992.fastq.gz	Fastqsanger.gz
	SRR5503851.fastq.gz	Fastqsanger.gz
	SRR5503852.fastq.gz	Fastqsanger.gz

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Identification of the Sulfolobus acidocaldarius small RNome and L7Ae-RNA interactome

Project description:Small RNAs and L7Ae co-immunoprecipitated RNA were isolated from the thermophilic archaeon Sulfolobus acidocaldarius and subjected to Illumina sequencing. The sRNome revealed a high abundance of C/D box sRNAs and CRISPR RNAs. The L7Ae-RNA interactome showed enrichment of all known interactors of the L7Ae protein, i.e. C/D box sRNAs, H/ACA box sRNAs, RNaseP, rRNA. A high abundance of reads for the SRP RNA was found, suggesting L7Ae´s interaction with this universal RNA. Many mRNA reads were enriched, several of them comprise binding sites for the L7Ae protein.

2017-02-10 | GSE94748 | GEO

Transcription profiling of hyperthermophilic crenarchaea Sulfolobus solfataricus and Sulfolobus acidocaldarius to UV irradiation

Project description:Analysis of transcriptional response to UV irradiation in two related crenarchaea, Sulfolobus solfataricus and Sulfolobus acidocaldarius.

2007-11-21 | E-MEXP-1252 | biostudies-arrayexpress

Transcriptome analysis of Sulfolobus acidocaldarius DSM 639 under sugar-free and sugar-rich conditions

Project description:Sulfolobus acidocaldarius is an obligate aerobe that grows in hot and acidic environments. S. acidocaldarius have been reported to grow on a variety of organic compounds as carbon and energy sources. However, little is known about systemic elucidation of carbon utilization for biomass formation and energy metabolism in S. acidocaldarius. In this analysis, the effect of glucose on genome-wide transcriptional profiling in S. acidocaldarius DSM 639 was investigated by RNA-Seq technology.

2020-11-15 | GSE107083 | GEO

Sulfolobus acidocaldarius phosphoproteome

Project description:Shotgun phosphoproteome of Sulfolobus acidocaldarius using PAciFIC technique. Briefly, proteins were denatured, alkylated, trypsine digestion, SCX separation Fractionated peptides were analysed on Bruker HCTultra. Data processing and bioinformatics: data from mass spectrometry were then extracted to mgf format using Bruker Data Analysis V4.0 with a MRM script, these were then were searched against the Sulfolobus acidocaldarius database (containing 2223 proteins) downloaded from NCBI in March 2010 using Phenyx V 2.6 (Genebio, Geneva). The searches were performed using parameters as follows: carbamidomethylation of cysteine (fixed modification), oxidation of methionine (variation), and phosphorylation of serine, tyrosine, threonine (variation), trypsin with 2 missed cleavages. Furthermore, other parameters such as parent, MS/MS, tolerances were set at 2.0 and 0.8 Da, respectively, whilst minimum peptide length, z-score, p-value and AC score were set at 5, 5.5, 10-5, and 5.5 respectively.

2014-04-15 | PXD000289 | Pride

The genome-scale DNA-binding of FadR transcription factor in Sulfolobus acidocaldarius

Project description:This is ChIPseq result of FadR, which is the only TetR family regulator presented in Sulfolobus acidocaldarius. The aim of the study is to gain insights into the function of TetR regulator by analyzing its whole genome binding sites.

2017-12-30 | E-MTAB-6328 | biostudies-arrayexpress

The genome-scale DNA-binding of FadR transcription factor in Sulfolobus acidocaldarius

Project description:This is ChIPseq result of FadR (Saci_1107), which is the only TetR family regulator presented in Sulfolobus acidocaldarius. The aim of the study is to gain insights into the function of TetR regulator by analyzing its whole genome binding sites.

2017-12-14 | GSE108039 | GEO

Sulfolobus acidocaldarius

Project description:Sulfolobus acidocaldarius Raw sequence reads

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Sulfolobus acidocaldarius

Project description:Sulfolobus acidocaldarius Genome sequencing and assembly

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Sulfolobus acidocaldarius SUSAZ

Project description:Sulfolobus acidocaldarius SUSAZ Genome sequencing

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Project description:Sulfolobus acidocaldarius: Transcriptome of RNase aCPSF2 Mutant