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Citrus reticulata

ABSTRACT: Expression data from a staygreen mutant of citrus and its wild type [RNA-Seq]

PROVIDER: PRJNA374493 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5249000.fastq.gz	Fastqsanger.gz
	SRR5249010.fastq.gz	Fastqsanger.gz
	SRR5249001.fastq.gz	Fastqsanger.gz
	SRR5249011.fastq.gz	Fastqsanger.gz
	SRR5249002.fastq.gz	Fastqsanger.gz

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Citrus reticulata

Project description:Expression data from a staygreen mutant of citrus and its wild type

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Transcriptome profiling for seed abortion in Ponkan (Citrus reticulata)

Project description:Seed developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying seed developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the seeds of seedless Ponkan ‘Huagan No.4’ (Citrus reticulata) (HG) and seeded Ponkan ‘Egan No.1’ (Citrus reticulata) (EG) into nucellus and integument/seed coat tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of EG, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of seed abortion.

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Transcriptome analysis of Citrus reticulata ovule early development based on laser capture microdissection

Project description:Ovule developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying ovule developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the ovules of seedless Ponkan ‘Huagan NO.4’ (Citrus reticulata) (MT) and seeded Ponkan ‘Egan NO.1’ (Citrus reticulata) (WT) into nucellus and integument tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of WT, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of ovule abortion.

2024-09-01 | GSE183639 | GEO

Comparative transcript profiling of gene expression between seedless Ponkan mandarin (Citrus reticulata Blanco) and its seedy wild type during floral organ development

Project description:Seedlessness is an important agronomic trait for citrus, and male sterility (MS) is one main cause of seedless citrus fruit. However, the molecular mechanism of citrus seedlessness remained not well explored. An integrative strategy combining SSH (suppression subtractive hybridization) library with cDNA microarray was employed to study the underlying mechanism of seedlessness of a Ponkan mandarin seedless mutant (Citrus reticulate Blanco). Screening with custom microarray, a total of 279 differentially expressed clones were identified, and 133 unigenes (43 contigs and 90 singletons) were obtained after sequencing. Gene Ontology (GO) distribution showed that the majority of differential genes involved in metabolic process, responded to stimulus and functioned as DNA/RNA binding, catalytic activity and oxidoreductase activity. Expression dynamics of some candidate clones revealed a gene encoding male sterility protein 2 was highly up-regulated, while several transcription factors (TFs) such as AP2/EREBP, MYB, WRKY, NAC and C2C2-GATA zinc-finger domain TFs were specifically down-regulated in the seedless mutant compared with the wild type. Our research highlighted some candidate pathways that participated in the citrus male gametophyte development and could be beneficial for seedless citrus breeding in the future. Flower buds from the seedless citrus mutant and its wild type were collected at four developmental stages: quaring stage (SF, about 20 DBF), medium bud stage (MF, about 10 DBF), flowers at full bloom stage (BF) and young ovaries of 2-3 days after flowering (OV). Total RNA extracted from the mutant and the wild type was hybridized to the array. The hybridization was performed in duplicate by dye swap.

2012-05-21 | E-GEOD-38094 | biostudies-arrayexpress

Comparative transcript profiling of gene expression between seedless Ponkan mandarin (Citrus reticulata Blanco) and its seedy wild type during floral organ development

2012-05-22 | GSE38094 | GEO

Citrus reticulata

Project description:Genome assembly row data of Citrus reticulata 'Unshiu' and Citrus reticulata 'Succosa'.

| PRJNA1064391 | ENA

Expression data of different hormone treatments from a staygreen mutant of citrus and its wild type

Project description:Expression data of different hormone treatments from a staygreen mutant of citrus and its wild type

| PRJNA453557 | ENA

Citrus reticulata

Project description:Identification of differentially expressed microRNAs from a male sterile Ponkan mandarin (Citrus reticulata Blanco) and its fertile wild type by small RNA and degradome sequencing

| PRJNA231900 | ENA

Citrus reticulata

Project description:Comparative transcript profiling of gene expression between seedless Ponkan mandarin (Citrus reticulata Blanco) and its seedy wild type during floral organ development

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Expression data from postharvest Ponkan (Citrus reticulata Blanco) fruit under cold storage

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2009-12-18 | GSE19543 | GEO

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