Project description:RNAseq transcriptional profiling of Drosophila brains from wildtype, and period loss-of-function animals with time points taken over two days.
Project description:RNAseq experimental data of cattle positive to MAP infection are compared to negative uninfected controls, with the purpose to find a small set of differentially expressed genes able to discriminate between infected animals in a preclinical phase. A signature of 10 transcripts that differentiate between potentially infected, but clinically healthy, animals belonging to paratuberculosis positive herds and negative unexposed animals has been identified and crossvalidated.
Project description:Macrophage subsets were sorted from the epididymal adipose tissue of chow- and high-fat diet-fed WT, and their transcriptomic profiles were performed using RNAseq. CD9-high adipose tissue progenitors were sorted from the epididymal adipose tissue of high-fat diet-fed WT and Ccr2-deficient animals, and their transcriptomic profiles were performed using RNAseq.
Project description:To investigate the mechanism behind drug synergism between MLN8237 and venetoclax we injected DoHH2 human tumor cells into SCID mice SQ, treated animals for 3 days, and harvested tumors for RNAseq.
Project description:Mutations in Bicaudaul C 1 (BICC1), evolutionary conserved RNA-binding protein, cause renal cysts in mice and humans. These renal cysts are reminiscent of Polycystic Kidney Disease (PKD). How BICC1 is involved in the pathogenesis of polycystic kidney disease is still unknown. Recent studies highlighted that HNF4A plays a role in the regulation of metabolic pathways deregulated in this renal disease. Moreover, Menezes et al. [2012, https://doi.org/10.1371/journal.pgen.1003053] showed that combined kidney inactivation of Hnf4a and Pkd1 in mice significantly worsened the cystic phenotype. Here we investigated whether the DNA binding and transcriptional activity of HNF4A might be deregulated in kidneys from Bicc1 mouse model of polycystic kidney disease. HNF4A, H3K27ac, H3K4me1 ChIPseq experiments were performed in kidneys from male and female Bicc1 WT and KO mice. From the contralateral kidneys of the same animals used for the ChIPseq experiments, the total RNA was extracted for gene expression profiling by RNAseq. The RNAseq data was used to support the ChIP-seq analysis and to reveal deregulated pathways in BICC1 mutants.
Project description:We performed single-nuclei RNAseq of Sprague Dawley rat area postrema and nucleus tractus solitarius brain samples from animals treated with cisplatin to identify cellular subtype specific changes in the neural transcriptome.
Project description:We performed single-nuclei RNAseq of Sprague Dawley rat area postrema and nucleus tractus solitarius brain samples from animals treated with GDF-15 to identify cellular subtype specific changes in the neural transcriptome.