Project description:The aim of the experiments was to determine the regulon of the Bacillus subtilis alternative sigma factor SigI. Biological relevance: To expand our knowledge about Bacillus subtilis transcriptional network under unfavorable conditions. Experimental workflow overview: Bacillus subtilis 168 trp+ (BaSysBio) was used as the genetic background. (i) sigI-rsgI knock-out, (ii) rsgI knock-out, and (iii) wr strains were cultured in LB medium to mid-exponential phase at 37°C and 52°C. Total RNA was isolated from 3 ml of the culture. rRNA was depleted from the samples with RiboMinus; subsequently RNA-seq libraries were prepared (Illumina compatible NEXTflex Rapid Directional RNA-Seq Kit, Bioo Scientific) and sequenced at the EMBL GeneCore facility. The experiment was performed in three independent replicates.

2018-06-26 | E-MTAB-6314 | biostudies-arrayexpress

Correlation between chemotherapy and intestinal flora evolution in non-small cell lung cancer

Project description:Interventions: Control group:None;Bacillus group :Live bacillus licheniformis capsule Primary outcome(s): Overall survival Study Design: Parallel

| 35000 | ecrin-mdr-crc

Correlation between immunotherapy and intestinal flora evolution in non-small cell lung cancer

Project description:Interventions: Control group:None;Bacillus group :Live bacillus licheniformis capsule Primary outcome(s): Overall survival Study Design: Parallel

| 34999 | ecrin-mdr-crc

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