Project description:<p>This study utilized neurologically normal control samples deposited in North American Brain Expression Consortium (NABEC). This top-level study, phs001300, makes available all phenotype data of the NABEC study participants. <br/>In addition, molecular data of six sub-studies are available through dbGaP: <ol> <li>NABEC Genome-Wide Genotyping - <a href="study.cgi?study_id=phs000249">phs000249</a></li> <li>NABEC Exome Sequencing - <a href="study.cgi?study_id=phs001301">phs001301</a></li> <li>NABEC CAGE Sequencing of Human Cerebral Frontal Cortex - <a href="study.cgi?study_id=phs001302">phs001302</a></li> <li>NABEC mRNA Sequencing of human Cerebral Frontal Cortex - <a href="study.cgi?study_id=phs001353">phs001353</a></li> <li>NABEC Total RNA Sequencing of human Cerebral Frontal Cortex - <a href="study.cgi?study_id=phs001354">phs001354</a></li> <li>NABEC Neurochip Genotyping - <a href="study.cgi?study_id=phs001462">phs001462</a></li> </ol> </p>
Project description:Cap analysis of gene expression (CAGE) and massive parallel sequencing were used to profile the promoterome of aged human brains from five regions, namely: caudate, frontal cortex, hippocampus, putamen and temporal cortex. 25 RNA libraries from post-mortem brain tissue (five caudate, five frontal, 5 hippocampus, 5 putamen, five temporal RNA libraries from seven individuals) were processed using CAGE protocol and CAGE tags derived from the 25 libraries were sequenced with Illumina.
Project description:Cap analysis of gene expression (CAGE) and massive parallel sequencing were used to profile the promoterome of aged human brains from five regions, namely: caudate, frontal cortex, hippocampus, putamen and temporal cortex.
Project description:This experiment contains the subset of data corresponding to human RNA-Seq data from experiment E-GEOD-30352 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30352/), which goal is to understand the dynamics of mammalian transcriptome evolution. To study mammalian transcriptome evolution at high resolution, we generated RNA-Seq data (∼3.2 billion Illumina Genome Analyser IIx reads of 76 base pairs) for the polyadenylated RNA fraction of brain (cerebral cortex or whole brain without cerebellum), cerebellum, heart, kidney, liver and testis (usually from one male and one female per somatic tissue and two males for testis) from nine mammalian species: placental mammals (great apes, including humans; rhesus macaque; mouse), marsupials (gray short-tailed opossum) and monotremes (platypus). Corresponding data (∼0.3 billion reads) were generated for a bird (red jungle fowl, a non-domesticated chicken) and used as an evolutionary outgroup.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
