Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA375878 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Exome sequencing of North American Brain Expression Consortium (NABEC) subject.
| EGAD00001002886 | EGA
Project description:NABEC: North American Brain Expression Consortium
| PRJNA375877 | ENA
Project description:NABEC (North American Brain Expression Consortium)
| PRJNA75637 | ENA
Project description:North American Brain Expression Consortium: DNA Methylation
| PRJNA164721 | ENA
Project description:North American Brain Expression Consortium and UK Human Brain Expression Database: Gene Expression
| PRJNA164719 | ENA
Project description:North American Brain Expression Consortium: Laser capture microdissection of Cerebellar Purkinje Cells
| PRJNA163855 | ENA
Project description:<p>This study utilized neurologically normal control samples deposited in North American Brain Expression Consortium (NABEC). This top-level study, phs001300, makes available all phenotype data of the NABEC study participants. <br/>In addition, molecular data of six sub-studies are available through dbGaP: <ol> <li>NABEC Genome-Wide Genotyping - <a href="study.cgi?study_id=phs000249">phs000249</a></li> <li>NABEC Exome Sequencing - <a href="study.cgi?study_id=phs001301">phs001301</a></li> <li>NABEC CAGE Sequencing of Human Cerebral Frontal Cortex - <a href="study.cgi?study_id=phs001302">phs001302</a></li> <li>NABEC mRNA Sequencing of human Cerebral Frontal Cortex - <a href="study.cgi?study_id=phs001353">phs001353</a></li> <li>NABEC Total RNA Sequencing of human Cerebral Frontal Cortex - <a href="study.cgi?study_id=phs001354">phs001354</a></li> <li>NABEC Neurochip Genotyping - <a href="study.cgi?study_id=phs001462">phs001462</a></li> </ol> </p>
| phs001300 | dbGaP
Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.
2019-07-31 | GSE114179 | GEO
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
2010-09-25 | GSE24338 | GEO
Project description:Kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5'-phosphate (PLP) dependent enzymes known as the aspartate aminotransferase superfamily or alpha-family. The Homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. The human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacterial expression system using Ni metal ion affinity chromatography. Kinetic characterization of the recombinant enzyme reveals classic Michaelis-Menten behavior, with a Km of 28.3 +/- 1.9 microM and a specific activity of 1.75 micromol min-1 mg-1 for 3-hydroxy-dl-kynurenine. Crystals of recombinant kynureninase that diffracted to 2.0 A were obtained, and the atomic structure of the PLP-bound holoenzyme was determined by molecular replacement using the Pseudomonas fluorescens kynureninase structure (PDB entry 1qz9) as the phasing model. A structural superposition with the P. fluorescens kynureninase revealed that these two structures resemble the "open" and "closed" conformations of aspartate aminotransferase. The comparison illustrates the dynamic nature of these proteins' small domains and reveals a role for Arg-434 similar to its role in other AAT alpha-family members. Docking of 3-hydroxy-l-kynurenine into the human kynureninase active site suggests that Asn-333 and His-102 are involved in substrate binding and molecular discrimination between inducible and constitutive kynureninase substrates.
| S-EPMC2531291 | biostudies-literature