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Drosophila melanogaster

ABSTRACT: The distribution of Brahma in the genome of Drosophila melanogaster S2 cells and the roles of Brahma in transcription and pre-mRNA processing

PROVIDER: PRJNA376457 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR5279380_1.fastq.gz	Fastqsanger.gz
	SRR5279380_2.fastq.gz	Fastqsanger.gz
	SRR5279381_1.fastq.gz	Fastqsanger.gz
	SRR5279381_2.fastq.gz	Fastqsanger.gz
	SRR5279382.fastq.gz	Fastqsanger.gz

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The distribution of Brahma in the genome of Drosophila melanogaster S2 cells and the roles of Brahma in transcription and pre-mRNA processing

Project description:We have carried out ChIP-seq to study the distribution of Brahma in the genome of S2 cells and to identify the Brahma-bound genes. We have also knocked down Brahma by RNA interference and we have carried out RNA-seq to determine the changes induced by Brahma depletion in the transcriptome of S2 cells. Furthermore, we have carried out RNA-seq experiments to profile S2 cells in which we first deplete Brahma by RNA interference and then express recombinant Brahma (either wild-type or ATPase mutant). These datasets are being used in several projects aimed at gaining understanding on the roles of Brahma in transcription regulation and pre-mRNA processing.

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