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Homo sapiens

ABSTRACT: The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 Acute Myelogenous Leukemia

PROVIDER: PRJNA377303 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5296590_1.fastq.gz	Fastqsanger.gz
	SRR5296590_2.fastq.gz	Fastqsanger.gz
	SRR5296600_1.fastq.gz	Fastqsanger.gz
	SRR5296600_2.fastq.gz	Fastqsanger.gz
	SRR5296591_1.fastq.gz	Fastqsanger.gz

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The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 Acute Myelogenous Leukemia

Project description:Acute Myeloid Leukemia (AML) with MLL gene rearrangements demonstrate unique gene expression profiles driven by MLL-fusion proteins. Here, we identify the circadian clock transcription factor SHARP1 as a novel oncogenic target in MLL-AF6 AML, which has the worst prognosis among all subtypes of MLL rearranged AMLs. SHARP1 is expressed solely in MLL-AF6 AML, and its expression is regulated directly by MLL-AF6 / DOT1L. Suppression of SHARP1 induces robust apoptosis of human MLL-AF6 AML cells. Genetic deletion in mice delays the development of leukemia and attenuated leukemia-initiating potential, while sparing normal hematopoiesis. Mechanistically, SHARP1 binds to transcriptionally active chromatin across the genome and activates genes critical for cell survival as well as key oncogenic targets of MLL-AF6. Our findings demonstrate the unique oncogenic role for SHARP1 in MLL-AF6 AML.

2018-04-26 | GSE95511 | GEO

Expression data from a human MLL-AF6 positive AML cell line

Project description:The MLL gene on chromosome 11 fuses to the AF6 gene on chromosome 6 in a balanced chromosomal translocation that is characetristic of certain adult and pediatric human leukemias. We established a murine leukemia model of MLL-AF6 using the retroviral MLL-AF6 contruct in a bone marrow transplantation system. The ML2 human MLL-AF6 positive leukemia cell line was used for gene expression profiling to assess the transctiptional profile in MLL-AF6 leukemias.

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Project description:MLL-AF6 leukemia

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Expression data from a human MLL-AF6 positive AML cell line

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Smith_AF6-RAS_2017

Project description:This dataset contains 14 raw mass spectrometry files associated with the study by Smith et al. that studies the evolution of AF6-RAS association and its implications in Mixed-Lineage Leukemia (MLL). Profiling of MLL and fusion proteins (with Afadin, AF6) was performed by BioID.

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High-throughput Sequencing Facilitates Quantitative Analysis of Bone Marrow Macrophages and Spleenic Macrophages MicroRNA Expression in mouse MLL-AF9 acute myelogenous leukemia

Project description:The goals of this study aim to reveal microRNA expression profiles of leukemia-associated macrophages and regulatory mechanism in response to the microenvironmental cues in mouse MLL-AF9 acute myelogenous leukemia

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Genome-wide analysis of gene expression in MLL translocation transformed mouse leukemia cell lines

Project description:The purpose of this study is to investigate the transcriptional programs as it relates to disease latency initiated by different MLL fusion proteins, including: MLL-AF1p, MLL-AF6, MLL-Gas7, MLL-AF9 and MLL-ENL. Leukemia cell lines were established by transforming kit+ mouse bone marrow cells with retroviruses coding MLL-AF1p, MLL-AF6, MLL-Gas7, MLL-AF9 or MLL-ENL. At early phase after the cell lines were established, cells growing at exponential phase (cell density at 0.5~1x106/ml) were harvested for RNA extraction and sequencing purpose.

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Expression data from MLL-AF6 positive leukemia cells

Project description:The MLL gene on chromosome 11 fuses to the AF6 gene on chromosome 6 in a balanced chromosomal translocation that is characetristic of certain adult and pediatric human leukemias. We established a murine leukemia model of MLL-AF6 using the retroviral MLL-AF6 contruct in a bone marrow transplantation system. Leukemic bone marrow cells from a sacrificed moribund mouse were thawed and RNA was extracted using TRIZOL (Invitrogen) and purified further using the RNAeasy kit (Qiagen) under standard conditions and and hybridized onto Affymetrix arrays.

2013-04-03 | E-GEOD-43067 | biostudies-arrayexpress

Genome wide mapping of histone 3 lysine 79 dimethylation in MLL rearranged and control human leukemia cell lines

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