Project description:To this date, host transcriptome studies in leprosy have focused on Schwann cells, as well as mouse-footpad and skin biopsies. Despite macrophages being the most infected cell types in leprosy lesions, there is no genome-wide experiments with this model. Here, we aimed at identifying host macrophages transcriptional changes induced by live-Mycobacterium leprae infection for 48 hours.
Project description:Our object is to characterize the distinguish gene enrichment group in skin of Mycobacterium leprae (M. leprae)-infected footpads compared to that of Mycobacterium leprae (M. leprae) non-infected footpads. One-condition experiment, Skin of M. leprae non-infected footpads (control) vs. Skin of M. leprae infected footpads (sample). Biological replicates: 3 control and 3 sample, independently grown and harvested from isolator. One replicate per array.
Project description:Our object is to characterize the distinguish gene enrichment group in skin of Mycobacterium leprae (M. leprae)-infected footpads compared to that of Mycobacterium leprae (M. leprae) non-infected footpads.
Project description:In an attempt to understand M. leprae interaction with the human host, Stanford Genomics HEEBO Arrays were use to identify modulated genes in primary human Schwann cells infected with live M. leprae at a MOI of 100:1 for 24 hours. Dual channel competitive hybridizations between M. leprae infected and non infected Schwann cells including 3 independent biological replicates and a technical replicate in the form of dye swap.
Project description:Mycobacterium leprae infect mouse Schwann cells in vitro, reprogram the Schwann cells, and induce mesenchymal stem cell-like cells. In this reprogramming, a vast number of stem cell-related genes, especially those with transcription factor activities are differentially regulated We used microarrays to detail the global program of gene expression underlying the Mycobacterial infection and identified distinct classes of differentially-regulated genes during this process.
Project description:Our goal is to understand the mechanism of granuloma formation in molecular level using Mycobacterium leprae (M. leprae)-infected footpads. One-condition experiment, M. leprae non-infected footpads (control) vs. M. leprae infected footpads (sample). Biological replicates: 6 control, 6 (sample), independently grown and harvested from isolator. One replicate per array.
Project description:In attempt to understand M.leprae interaction with the human host, Applied Biosystems microarrays containing 30,865 probles were used to identify modulated genes in primary human Schwann Cells (SC) infected with live M. leprae at two early time points, 24 and 48 hours. A total of 4 independent experimental samples were prepared which were hybridized to two replicate microarrays each.The four experimental samples included both uninfected and M. leprae infected Schwann cells at both 24 and 48 hours.
Project description:Mycobacterium leprae, the causative agent of leprosy, an obligate intracellular pathogen has the ability to survive and grow for extended periods within phagocytes and Schwann cells. M. leprae genome analysis predicts a highly degraded genome resulting in a significant loss of its genomic coding capacity. Detailed dynamics of carbon sources for energy utilization and growth of M. leprae is unclear. This study, therefore, presents M. leprae transcriptome during in vivo growth and ex vivo stationary phases, and explores metabolic pathways relevant to its growth from global gene expression data. This report provides a glimpse of some of M. leprae nutritional requirements for growth, which most likely, needs to be supplemented, in an axenic growth media.
Project description:In an attempt to understand M. leprae interaction with the human host, Stanford Genomics HEEBO Arrays were use to identify modulated genes in primary human Schwann cells infected with live M. leprae at a MOI of 100:1 for 24 hours.
Project description:Our goal is to understand the mechanism of granuloma formation in molecular level using Mycobacterium leprae (M. leprae)-infected footpads.
