Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tetrahymena thermophila

ABSTRACT: N6-adenine DNA methylation in Tetrahymena

PROVIDER: PRJNA378886 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5337751_subreads.fastq.gz	Fastqsanger.gz
	SRR5337752_1.fastq.gz	Fastqsanger.gz
	SRR5337752_2.fastq.gz	Fastqsanger.gz
	SRR5337753_subreads.fastq.gz	Fastqsanger.gz
	SRR5337754_subreads.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Tetrahymena thermophila

Project description:Semi-conservative transmission of DNA N6-adenine methylation in a unicellular eukaryote

| PRJNA932808 | ENA

Tetrahymena thermophila

Project description:DNA elimination analysis of scnRNA production-defective Tetrahymena

| PRJNA282768 | ENA

DNA Methylation in Tetrahymena Thermophila

Project description:DNA methylation comparison between early growing Tetrahymena Thermophila and late growing Tetrahymena Thermophila

| PRJEB27686 | ENA

Tetrahymena thermophila

Project description:Tetrahymena thermophila

| PRJNA11958 | ENA

Tetrahymena thermophila

Project description:DNA elimination analysis of Tetrahymena cells defective in heterochromatin body formation

| PRJNA287581 | ENA

Analysis of conjugation-specific 26-32nt siRNAs of Tetrahymena thermophila

Project description:Distinct classes of small RNAs are often selectively sorted to different Argonaute proteins. Various properties of small RNAs, such as length, terminal nucleotide, thermodynamic asymmetry and duplex mismatches, can impact sorting in different RNA silencing pathways in diverse eukaryotes. The developmentally regulated ~26-32 nt siRNAs, which are involved in programmed DNA elimination in Tetrahymena, show a strong bias for uracil at the 5' end. In this study, we analyzed loaded and unloaded populations of ~26-32 nt siRNAs by deep RNA sequencing. We show that the production process is the main determinant of size, whereas the 5' uracil bias is attributed not only to the process of loading siRNAs into the Argonaute protein Twi1p but also significantly to the initial processing of the siRNAs. We also show that both the loaded and the unloaded ~26-32 nt siRNAs have a strong bias for adenine as the 3rd base from the 3' terminus, suggesting that most of these siRNAs are direct Dicer products and little post-processing amplification of this class of siRNAs occurs. Further, we demonstrate that the siRNA-loading process in vivo can be deduced from the fraction of siRNAs with uracil as the first base. These findings provide biochemical bases for the attributes of ~26-32 nt siRNAs, which should help improve our understanding of their production and turnover in vivo. Examination of siRNA populations in wild-type and TWI1 KO Tetrahymena cells

2012-10-01 | E-GEOD-37696 | biostudies-arrayexpress

Tetrahymena thermophila

Project description:Tetrahymena thermophila Raw sequence reads

| PRJNA310376 | ENA

Tetrahymena thermophila

Project description:Tetrahymena thermophila Raw sequence reads

| PRJNA593714 | ENA

Tetrahymena thermophila

Project description:Tetrahymena thermophila Raw sequence reads

| PRJNA611686 | ENA

Tetrahymena thermophila

Project description:Tetrahymena thermophila Raw sequence reads