Project description:To investigate the long noncoding RNA (lncRNA) expression profiles in human apheresis platelet during the storage, and predict the potential biological function of lncRNAs in the process of platelet storage lesion. LncRNAs profiles of platelets were tested by Agilent Human lncRNA Array at 2nd, 5th, and 8th day during storage. Seven lncRNAs and four mRNAs were chosen to validate by real-time PCR, the result was consistent with the microarray. Bioinformatics method was applied to predict the function of lncRNAs.
Project description:To investigate the microRNAs (miRNAs) expression profiles in human apheresis platelet under platelet storage lesion (PSL), and predict the potential biomarkers in the process of PSL. miRNAs profiles of platelets were tested by illumina HiSeq2500 between PSL and nonPSL. 13 miRNAs were chosen to validate by real-time PCR, the result was consistent with the sequencing results.
Project description:To determine the transcriptomic (mRNA and microRNA) content present in fresh apheresis platelets and freeze dried platelets (Thrombosomes) we used RNA-Seq approach. The goal was to determine if the freeze drying process maintained the transcriptomic cargo of the fresh platelets.
Project description:Platelets are blood cells who play critical roles in numerous biological and disease processes. This study was designed to identify lncRNAs that may play a role in platelet reactivity. In this study, by using large-scale deep sequencing, we determined the expression profiles of lncRNAs in both hyperreactive and hyporeactive human platelets. To determine the potential link between the expression of lncRNAs and the function of platelets, the expression profiles of hyperreactive and hyporeactive platelets were compared. Compared with hyperreactive platelets, deep sequencing analysis demonstrated that differential lncRNA expression was a remarkable characteristic in hyporeactive platelets.
Project description:Investigation of miRNA expression level changes of human platelets stored for 2, 5 and 8 days，and to explore the mechanism of storage lesions of human platelets.
Project description:Human platelets collected from healthy donors according to standard international protocols were pooled and stored at 4ºC during 7 days. The small RNA population changes across the days were evaluated by NGS aproach. The smallRNA population was evaluated at fresh platelets and after 1 day, 2 days, 3 days, 4 days, 5 days and 7 days of storage at 4ºC.
Project description:In this project, the protein acetylation levels in human platelets and in WT and SIRT3-/- mouse platelets during storage were examined.
