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HTS-Seq analysis of Kdm4a wildtype and knockout females at 2.5 dpc

ABSTRACT: HTS-Seq analysis of Kdm4a wildtype and knockout females at 2.5 dpc

PROVIDER: PRJNA379371 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5341920.fastq.gz	Fastqsanger.gz
	SRR5341930.fastq.gz	Fastqsanger.gz
	SRR5349400.fastq.gz	Fastqsanger.gz
	SRR5349410.fastq.gz	Fastqsanger.gz
	SRR5349420.fastq.gz	Fastqsanger.gz

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Project description:Purpose: ChipSeq allows us to identify binding sites of target proteins of interest in the genome, helping create a global profile for the same. Kdm4a is a H3K9me3 histone demethylase that acts to prevent spurious accumulation of this repressive mark at H3K4me3 positive transcription start sites. To complement transcriptome data from Kdm4a mice, we performed Chip-Seq on 2.5dpc mouse uterus of 8-10 week old littermate control and Kdm4a knockout mice for Kdm4a, H3K9me3 and H3K4me3. This would help us check which of the target genes with changed gene expression was due to the direct change in histone lanscape in absence of Kdm4a.

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HTS-Seq analysis of Kdm4a wildtype and knockout females at 2.5 dpc

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:Purpose: We performed RNA-Seq on 2.5dpc mouse hypothalamus, pituitary, ovary and uterus of 8-10 week old littermate female control and Kdm4a knockout mice. Upon observing for the extent of altered genes of physiological relevance to observed female infertility phenotype, this would help us narrow down the most relevant tissue to perform genome wide ChipSeq binding profiles for Kdm4a, H3K4me3 and H3K9me3. This dataset would present likely target genes under direct control of Kdm4a.

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