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Strongylocentrotus purpuratus

ABSTRACT: The sea urchin larval response to immune challenge with Vibrio diazotrophicus

PROVIDER: PRJNA380184 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5367600.fastq.gz	Fastqsanger.gz
	SRR5367601.fastq.gz	Fastqsanger.gz
	SRR5367602.fastq.gz	Fastqsanger.gz
	SRR5367599.fastq.gz	Fastqsanger.gz

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The sea urchin larval response to immune challenge with Vibrio diazotrophicus

Project description:Seawater exposure to the gram negative marine bacterium Vibrio diazotrophicus induces a robust cellular response in sea urchin larvae that includes the migration of pigment cells to the gut epithelium, changes in cell behavior and altered gut morphology (Ho et al., 2016; PMID 27192936). To investigate the transcriptional underpinnings of this response, whole transcriptome sequencing was performed on mRNA isolated from larval samples collected at 0, 6, 12 and 24 hr of exposure to V. diazotrophicus. The morphological simplicity of the sea urchin larva provides a systems-level model for identifying biologically relevant transcriptional state changes in response to dysbiosis in the gut lumen.

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Project description:Gene expression in tissues from an immune challenged adult sea urchin (Strongylocentrotus purpuratus)

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Response to elevated pCO2 in Strongylocentrotus purpuratus larvae

Project description:Transcriptional profiling of purple sea urchin (Strongylocentrotus purpuratus) larvae cultured under three different seawater CO2 concentrations 400, 800, 1200 M-BM-5atm. The goal was to determine the effects of CO2, an important climate change variable, on global gene expression Larvae were cultured under three different seawater CO2 concentrations 400, 800, 1200 M-BM-5atm, each with four replicate cultures, and sampled at two developmental stages (gastrula and pluteus)

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DNA hydroxymethylation profiling of purple sea urchin (Strongylocentrotus purpuratus), european lancelet (Branchiostoma lanceolatum) and zebrafish (Danio rerio) using hMeDIP-seq

Project description:Using hMeDIP-seq we validated the single-base resolution hydroxymethylomes (ACE-seq) of sea urchin, lancelet and zebrafish embryos.

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Project description:A high-resolution transcriptome map of the sea urchin embryo

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Base-resolution DNA hydroxymethylation maps of purple sea urchin (Strongylocentrotus purpuratus), european lancelet (Branchiostoma lanceolatum) and zebrafish (Danio rerio) using ACE-seq

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