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Mus musculus

ABSTRACT: Epididymal adipose tissue gene expression in wildtype mice and mice with inactivated mesoderm specific transcript

PROVIDER: PRJNA380237 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5368680.fastq.gz	Fastqsanger.gz
	SRR5368681.fastq.gz	Fastqsanger.gz
	SRR5368682.fastq.gz	Fastqsanger.gz
	SRR5368673.fastq.gz	Fastqsanger.gz
	SRR5368683.fastq.gz	Fastqsanger.gz

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Epididymal adipose tissue gene expression in wildtype mice and mice with inactivated mesoderm specific transcript

Project description:The goal of this study is to compare transcriptome profiles of epididymal adipose tissue from wildtype mice and mice with a targeted inactivation of mesoderm specific transcript (Mest) fed a high fat diet from 8 to 16 weeks of age to gain insight for MEST function. Gene expression profiling resulted in relatively few changes (~36 targets; FDR padj<0.1) in gene expression in epididymal WAT from wildtype mice and mice with inactivated paternal allele for Mest. A less stringent analyses of the dataset (P ≤ 0.05; with mean of ≥ 50 sequence tags for at least one group) identified 395 genes more highly expressed in epididymal adipose tissue of wildtype mice and 301 genes more highly expressed in mice with a paternal inactivation of Mest. Gene pathway analyses of differentially expressed genes showed decreased expressed of genes associated with immune response and inflammation in high fat diet-fed mice with inactivated Mest compared with wildtype mice. Transcritome analyses supported phenotypic measurements which indicated improved glucose homeostasis in mice with inactivated Mest.

2017-08-17 | GSE96968 | GEO

The Nuclear Receptor Rev-erb alpha Regulates Adipose Tissue-Specific FGF21 Signaling (Microarray)

Project description:To investigate the role of the transcriptional repressor Rev-erb alpha in epididymal white adipose tissue, we performed a microarray analysis of gene expression in the epididymal white adipose tissue of wildtype and Rev-erb alpha knock-out mice. Examination of the transcriptome in epididymal white adipose tissue of Rev-erb alpha kockout mice compared to wildtype mice.

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Project description:The goal of this study is to identify the cistrome of the transcriptional repressor Rev-erb alpha in epididymal white adipose tissue. Performing Rev-erb alpha ChIP-seq on epididymal white adipose tissue from wildtype mice at 5PM when Rev-erb alpha protein level peaks in wild type (WT) mice, we were able to globally determine the genomic regions undergoing Rev-erb alpha-dependent de-repression. Examination of Rev-erb alpha binding in epididymal white adipose tissue.

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Project description:Expression data from mice epididymal adipose tissue

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The Nuclear Receptor Rev-erb alpha Regulates Adipose Tissue-Specific FGF21 Signaling (ChIP-seq, eWAT)

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Project description:Analysis of murine epididymal adipose tissue

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Project description:Epididymal Adipose Tissue Profiling In HMDP

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Transcriptome differences between brown adipose tissues of wildtype and UCP1-KO mice

Project description:We analyzed coding transcript abundance in murine, interscapular brown adipose tissue from wildtype and UCP1-KO mice.

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