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NSD2 negatively regulate innate immune response through Zmynd11

ABSTRACT: NSD2 negatively regulate innate immune response through Zmynd11

PROVIDER: PRJNA381190 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5398050.fastq.gz	Fastqsanger.gz
	SRR5398060.fastq.gz	Fastqsanger.gz
	SRR5398070.fastq.gz	Fastqsanger.gz
	SRR5398080_1.fastq.gz	Fastqsanger.gz
	SRR5398080_2.fastq.gz	Fastqsanger.gz

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NSD2 negatively regulate innate immune response through Zmynd11

Project description:This SuperSeries is composed of the SubSeries listed below.

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Genome-wide analysis of H3.3, H3K36me2, H3K36me3 and ZMYND11 accumulation in WT MEFs and NSD2 KO MEFs.

Project description:We found that the incorporation of histone H3 variant H3.3 was impaired and the accumulation of ZMYND11, which specifically binds to H3.3K36me3, was decreased in NSD2 KO MEFs. About H3K36me2, the average gene body profiles of H3K36me2 showed preferential enrichment of H3K36me2 at the promoter and first half of the genic regions. We examined H3K36me2 mark in super enhancer regions using the ROSE super enhancer prediction program and identified of the 519 super enhancer islands of H3K36me2 in WT MEFs the H3K36me2 signals were decreased in NSD2 KO MEFs

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Project description:NSD2 (also known as Whsc1, MMSET) play a role in H3K36 methyl transferase. Here we showed that 199 ISGs of the total 393 ISGs after 2h IFN treatment were up regulated in NSD2 KO MEFs, suggesting negatively regulate ISGs induction. IFN pretreatment creates transcriptional memory. In this study we identified 129 ISGs were memory ISGs and 40 ISGs were non-memory ISGs.

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Stable over expression of the SPOP-substrate ZMYND11 in VCaP cells

Project description:SPOP is known to bind to serine/theronine-rich degrons on substrate proteins. We identified two degron sequences within the PWWP and MYND domain of ZMYND11, a novel SPOP substrate, and investigated whether ZMYND11 may contribute to the transcriptional output of mutant SPOP in VCaP cancer cells. Hence, we performed over-expression of degron-deficient ZMYND11 and degron-deficient ZMYND11 with W294A mutation in VCaP cells. While WT ZMYND11 constructs can be robustly over-expressed at the mRNA level, only the degron-deficient variants yielded a robost increase in ZMYND11 protein expression. Degron mutant-induced transcriptional perturbations partially mimicked SPOP mutant-induced gene expression changes, suggesting that ZMYND11 is sufficient to recapitulate certain features of mutant SPOP.

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Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding

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Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity

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