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Arabidopsis thaliana

ABSTRACT: ENAP1 is involved in ethylene response

PROVIDER: PRJNA381206 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5398680.fastq.gz	Fastqsanger.gz
	SRR5398675.fastq.gz	Fastqsanger.gz
	SRR5398676.fastq.gz	Fastqsanger.gz
	SRR5398677.fastq.gz	Fastqsanger.gz
	SRR5398678.fastq.gz	Fastqsanger.gz

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Arabidopsis thaliana

Project description:ENAP1 is involoved in the EIN3- mediated ethylene response

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ENAP1 is involved in ethylene response

Project description:We report that ethylene regulate the ENAP1 binding. We perform a dual cross-linking procedure using the additional cross-linker ethylene glycol bis-succinimidylsuccinate (EGS; Thermo Scientific) along with formaldehyde according to (Pedmale et al. 2016) for Chip-sequencing (ChIPseq) of ENAP1, as well as ENAP1 ChIP- EIN2 reChIPseq (ChIP-reChIPseq), which were used chromatins isolated from 3-day old etiolated Col-0 seedlings treated with ethylene or air gas. Results show that ENAP1 is involved in the EIN2 dependent ethylene response.

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Project description:ENAP1 is involoved in the Histone H3K23 acetylation- and EIN3- mediated ethylene response

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Project description:We report that ethylene reduce the ENAP1 binding. We perform Chip-sequencing of ENAP1 using chromatins isolated from 3-day old etiolated ein3eil1 seedlings treated with ethylene or air gas. Results show that ethylene reduce ENAP1 binding in TSS region of EIN3 targeted genes that are regulated at the transcriptional level by ethylene.

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ENAP1 is involoved in the Histone H3K23 acetylation- and EIN3- mediated ethylene response

Project description:We report that ethylene reduce the ENAP1 binding. We perform Chip-sequencing of ENAP1 using chromatins isolated from 3-day old etiolated Col-0/ein2/ein3eil1 seedlings treated with ethylene or air gas. Results show that ethylene reduce ENAP1 binding in TSS region of EIN3 targeted genes that are regulated at the transcriptional level by ethylene.

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Project description:Histone deacetylase SRT2 mediates ethylene-induced transcriptional repression through interaction with ENAP1

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Project description:Genome-wide study reveals dual function of histone binding protein ENAP1 in ethylene signaling

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Project description:Genome-wide study reveals dual function of histone binding protein ENAP1 in ethylene signaling in ein3eil1 mutant plants

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Genome-wide study reveals dual function of histone binding protein ENAP1 in ethylene signaling in ein3eil1 mutant plants

Project description:Purpose: Next-generation sequencing (NGS) has been used to study the gene expression in different samples under air and ethylene treatment. The goal of this study is to uncover how ENAP1 and H3K23Ac dynamically coordinate with EIN3 to regulate gene expression in response to ethylene.

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