Ontology highlight
ABSTRACT:
PROVIDER: PRJNA381513 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR5438743.fastq.gz | Fastqsanger.gz | |||
| SRR5438744.fastq.gz | Fastqsanger.gz | |||
| SRR5438745.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 3 of 3 |
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Project description:Global warming is strongly affecting the Maritime Antarctica climate and the consequent melting of perennial snow and ice covers resulted in increased colonization by plants. Colobanthus quitensis is a vascular plant highly adapted to the harsh environmental conditions of Antarctic Peninsula and understanding how the plant is responding to global warming is a new challenging target for modern cell physiology. To this aim, we performed differential proteomic analysis on C. quitensis plants grown in natural conditions compared to plants grown for one year inside Open Top Chambers (OTCs) which determine an increase of about 4 °C at midday, mimicking the effect of global warming. A thorough analysis of the up- and down-regulated proteins highlighted an extensive metabolism reprogramming leading to the hypothesis that (i) photorespiration could play an important role in reducing ROS-mediated photodamage improving OTC plants protection against photoinhibition; (ii) OTC plants activate stronger antioxidant defenses as confirmed by enzymatic activity and TBARS content measurement; (iii) the deposition of cell wall components is impaired in OTC plants due to the down-regulation of many enzymes involved in their biosyn-thesis; (iv) OTC plants could suffer of a reduced freezing tolerance due to lower concentration of soluble sugars acting as osmoprotectants.
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Project description:Due to the progressive environmental change that the Antarctic Peninsula has undergone over time, a more comprehensive overview of the metabolic features of Colobanthus quitensis becomes particularly interesting to assess its ability to respond to environmental stresses. To this end, a differential proteomic approach has been used to study the response of C. quitensis to different environmental cues.
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Project description:Two fecal samples from humpback whales (Megaptera novaeangliae) off the West Antarctic Peninsula were analyzed on 21 Tesla FT-ICR MS to characterize the metal-binding ligand pool that is released into seawater. Also part of this project is MassIVE deposit MSV000094994. This work was conducted at the The National High Magnetic Field Laboratory, which is supported by the National Science Foundation Division of Materials Research and Division of Chemistry through DMR-1644779 and the State of Florida
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Project description:Glaciers are populated by a large number of microorganisms including bacteria, archaea and microeukaryotes. From an ecological point of view, three ecosystems can be differentiated in glaciers: the supraglacial ecosystem, the subglacial ecosystem and the englacial ecosystem. Several factors such as solar radiation, nutrient availability and water content greatly determine the diversity and abundance of microbial populations, the type of metabolism and the biogeochemical cycles. This huge diversity is threatened by climate change. This sampling place, Sarrios ice cave (Monte Perdido massif, Spanish Pyrenees) host one of the last permafrost deposits. Permafrost can provide us very worthy information about past climate and environmental conditions, therefore it should be studied as soon as possible due to the huge retreat that is already taking place. In order to study their metabolic potentials, samples of ice and permafrost were taken from an Antarctic glacier. The cells were harvested and their proteins were extracted and analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI/TOF/TOF). Several proteins and enzymes were found that demonstrate the existence of cellular activity at subzero temperatures. In this way it is shown that the englacial microorganisms are not quiescent, but that they maintain an active metabolism and play an important role in the glacial microbial community.
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