Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gossypium arboreum cultivar:Shixiya-1

ABSTRACT: Gossypium arboreum cultivar:Shixiya-1 Genome sequencing

PROVIDER: PRJNA382310 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5451034_subreads.fastq.gz	Fastqsanger.gz
	SRR6217761_1.fastq.gz	Fastqsanger.gz
	SRR6217761_2.fastq.gz	Fastqsanger.gz
	SRR6217784_1.fastq.gz	Fastqsanger.gz
	SRR6217784_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Gossypium arboreum cultivar:shixiya1

Project description:Gossypium arboreum cultivar:shixiya1 Genome sequencing

| PRJNA614615 | ENA

Gossypium arboreum cultivar:xx

Project description:Gossypium arboreum cultivar:xx Genome sequencing

| PRJNA381999 | ENA

Gossypium arboreum

Project description:Gossypium arboreum Genome sequencing

| PRJNA203682 | ENA

Gossypium arboreum cultivar:DLSa17

Project description:Gossypium arboreum cultivar:DLSa17 Genome sequencing and assembly

| PRJNA275198 | ENA

Gossypium arboreum cultivar:zb1

Project description:Gossypium arboreum cultivar:zb1 Genome sequencing and assembly

| PRJNA935667 | ENA

Gossypium arboreum cultivar:Shixiya1

Project description:Gossypium arboreum cultivar:Shixiya1 Raw sequence reads

| PRJNA312425 | ENA

Gossypium arboreum

Project description:Gossypium arboreum Genome sequencing and assembly

| PRJNA203683 | ENA

Gossypium arboreum

Project description:Gossypium arboreum Assembly

| PRJNA614486 | ENA

High-throughput sequencing of small RNAs in Gossypium arboreum

Project description:Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from different Gossypium arboreum tissues (leaves, flowers and fibers). The high depth of the resulting datasets enabled us to examine in detail critical small RNA features as size distribution, tissue-specific regulation and sequence conservation between different organs in this species. We also developed database resources and a dedicated website (http://smallrna.udel.edu/) with computational tools for allowing other users to identify new miRNAs or siRNAs involved in specific regulatory pathways, verify the degree of conservation of these sequences in other plant species and map small RNAs on genes or larger regions of the maize genome under study. Small RNA libraries were derived from leaves, flowers and fibers of Gossypium arboreum. Each tissue represented a mixture of developmental stages. Total RNA was isolated using the Plant RNA Purification Reagent (Invitrogen) and submitted to Illumina (Hayward, CA, http://www.illumina.com) for small RNA library construction using approaches described in (Lu et al., 2007) with minor modifications. The small RNA libraries were sequenced with the Sequencing-By-Synthesis (SBS) technology by Illumina. PERL scripts were designed to remove the adapter sequences and determine the abundance of each distinct small RNA. We thank Thea Wilkins for providing the plant material and Kan Nobuta and Gayathri Mahalingam for assistance with the computational methods..

2011-05-09 | E-GEOD-28965 | biostudies-arrayexpress

Gossypium arboreum cultivar:AKA8401

Project description:Gossypium arboreum cv. AKA8401 Genome sequencing

| PRJNA181159 | ENA