Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus

ABSTRACT: RNA expression in anterior nasal septum cartilage of wildtype and Fgfr2+/S252W Apert syndrome model mice at embryonic day (E)14.5.

PROVIDER: PRJNA382979 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5448773_1.fastq.gz	Fastqsanger.gz
	SRR5448773_2.fastq.gz	Fastqsanger.gz
	SRR5448774_1.fastq.gz	Fastqsanger.gz
	SRR5448774_2.fastq.gz	Fastqsanger.gz
	SRR5448775_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

RNA expression in anterior nasal septum cartilage of wildtype and Fgfr2+/S252W Apert syndrome model mice at embryonic day (E)14.5.

Project description:The nasal capsule cartilage of Apert Fgfr2+/S252W mice increases in size compared to wildtype cartilage during embryonic craniofacial development. Increased levels of markers of cell proliferation can be detected in the Apert Fgfr2+/S252W anterior nasal septum cartilage compared to wildtype at E14.5. We have performed laser capture microdissection of this cartilage in these genotypes at this age to generate RNA-Seq libraries and compare gene expression. Expression analysis shows increased expression of genes related to chromosome condensation, nuclear division, and the cell cycle in Apert Fgfr2+/S252W anterior nasal septum cartilage compared to wildtype.

2018-09-25 | GSE97828 | GEO

Nasal septum deviation as the consequence of BMP-controlled changes to cartilage properties

Project description:Nasal septum deviation (NSD) is a common abnormality of the septal cartilage often associated with disordered breathing. The etiology of NSD is unknown. BMP7 neural crest-knockout mice, a well-characterized model for midfacial hypoplasia and nasal airway obstruction, develops a deviated septum by 4 weeks of age. Using comparative gene expression, quantitative proteomics, and immunofluorescence analysis we investigated the septum prior, immediately preceding, and with established deviation. We provide a detailed description of cellular and molecular changes leading to septum deviation, including changes to extracellular matrix organization, cell metabolism, and chondrocyte differentiation. Loss of BMP7 was associated with acquisition of elastic cartilage properties, a switch to glucose metabolism, and molecular characteristics commonly associated with osteoarthritis (OA). Many alterations preceded the deviation establishing that molecular changes to chondrocyte properties predispose to the deviation. Interestingly, NSD was associated with a persistent increase in BMP2. Genetic reduction of BMP2 in BMP7ncko mice restores WNT signalling, energy metabolism, and rescues NSD, showing the importance of balanced BMP signaling for normal cartilage. Many of the cellular and molecular changes have been described for knee osteoarthritis, suggesting that these two pathologies might have a similar underlying etiology.

2021-04-30 | PXD024813 | Pride

Single-cell RNA sequencing analysis of E14.5 mouse skin data

Project description:Mouse embryo skin samples were collected around embryonic day 14.5 (E14.5). Cells from the wildtype and the Wls mutant were pooled for analyses.

2024-02-15 | GSE255534 | GEO

Whole kidneys at embryonic day 14.5: wildtype (WT) versus Hoxb7Cre transgene mediated conditional Mdm4 knockout kidneys

Project description:Whole kidneys at embryonic day 14.5: wildtype (WT) versus Hoxb7Cre transgene mediated conditional Mdm4 knockout kidneys

| PRJNA574125 | ENA

RNA-seq of mouse barrelette neurons at embryonic day 14.5

Project description:RNA-seq of mouse barrelette neurons at embryonic day 14.5

| PRJEB34317 | ENA

Bulk RNA-seq analysis of developing ocular anterior segments from wildtype and Col4a1 mutant mice

Project description:COL4A1 mutations cause Gould syndrome, a multi-system disorder which is characterized by a broad range of cerebrovascular, ocular, muscular, renal, or cardiac manifestations. To investigate the patheogenic mechanism of Col4a1 mutations in ocular anterior segment dysgenesis, we used a Col4a1 mutant mouse model that recapitulate many aspects of the pathophysiological hallmarks of Gould syndrome including anterior segment dysgenesis. We then isolated developing anterior segments from wildtype and mutant mice and performed differntial expression analysis using bulk RNAseq.

2022-05-18 | GSE202801 | GEO

Analysis of transcriptome changes in Kmt2d deletion in cardiac mesoderm, anterior heart field precursors and cardiomyocytes

2015-11-04 | GSE74679 | GEO

NeuroD2 ChIP-SEQ from embryonic cortex

Project description:NeuroD2 targets were identified from embryonic day 14.5 cerebral cortex tissue.

2015-09-01 | GSE67539 | GEO

Analysis of transcriptome changes in Kmt2d deletion in cardiac mesoderm, anterior heart field precursors and cardiomyocytes

Project description:KMT2D is required in the cardiac mesoderm, anterior heart field precursors and cardiomyocytes. Kmt2d deletion in cardiac mesoderm (Mesp1Cre) is embryonic lethal at E10.5 and mutants have hypoplastic hearts; Kmt2d deletion in anterior heart field precursors (Mef2cAHF::Cre) deletion is embryonic lethal at E13.5 and mutants have defects in septation of outflow tract and interventricular septum (IVS); Kmt2d deletion in cardiomyocytes (Tnnt2::Cre) deletion is embryonic lethal at E14.5 and mutants have defects in IVS septation and compact myocardium. The goal of this study is to compare changes in gene expression in these Kmt2d conditional deletion mutants and understand common or distinct pathways dysregulated in absence of KMT2D. Whole genome gene expression analysis was performed on RNA isolated from control and mutant embryonic hearts (or right ventricles and outflow tract for anterior heart field deletion samples). Libraries were prepared using Illumina TruSeq Paired-End Cluster Kit v3, and sequenced with the Illumina HiSeq 2500 system for pair-ended 100 base pairs (PE 100 bp).

2015-11-04 | E-GEOD-74679 | biostudies-arrayexpress

Mus musculus

Project description:Genome wide mapping of H3K27me3 in Liver at embryonic day 14.5

| PRJDB1795 | ENA