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Homo sapiens

ABSTRACT: Small RNA-Seq analysis of human lung fibroblasts exposed to TGF-β

PROVIDER: PRJNA383014 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5448900.fastq.gz	Fastqsanger.gz
	SRR5448895.fastq.gz	Fastqsanger.gz
	SRR5448896.fastq.gz	Fastqsanger.gz
	SRR5448897.fastq.gz	Fastqsanger.gz
	SRR5448898.fastq.gz	Fastqsanger.gz

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Homo sapiens

Project description:RNA-Seq analysis of human lung fibroblasts exposed to TGF-β

| PRJNA383015 | ENA

Small RNA-Seq analysis of human lung fibroblasts exposed to TGF-β

Project description:To assess the transcriptomic response of human lung fibroblast to TGF-β, human MRC5 pulmonary fibroblasts (CCL-171) were exposed to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Three independent experiments were carried out.

2019-04-11 | GSE97832 | GEO

RNA-Seq analysis of human lung fibroblasts exposed to TGF-β

Project description:To assess the transcriptomic response of human lung fibroblast to TGF-β, human MRC5 pulmonary fibroblasts (CCL-171) were exposed or not to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Three independent experiments were carried out.

2019-04-11 | GSE97829 | GEO

Bi-directional epithelial-mesenchymal crosstalk provides self-sustaining pro-fibrotic signals in pulmonary fibrosis

Project description:Idiopathic pulmonary fibrosis (IPF) is the prototypic progressive fibrotic lung disease with a median survival of 2-4 years. Injury to and/or dysfunction of alveolar epithelium are strongly implicated in IPF disease initiation, but what factors determine why fibrosis progresses rather than normal tissue repair occurs remain poorly understood. We previously demonstrated that ZEB1-mediated epithelial-mesenchymal transition (EMT) in human alveolar epithelial type II (ATII) cells augments TGF-β-induced profibrogenic responses in underlying lung fibroblasts by paracrine signalling. Here we investigated bi-directional epithelial-mesenchymal crosstalk and its potential to drive fibrosis progression. RNA sequencing (RNA-seq) of lung fibroblasts exposed to conditioned media from ATII cells undergoing RAS-induced EMT identified many differentially expressed genes including those involved in cell migration and extracellular matrix (ECM) regulation. We confirmed that paracrine signalling between AS-activated ATII cells and fibroblasts augmented fibroblast recruitment and demonstrated that this involved a ZEB1-tissue plasminogen activator (tPA) axis. In a reciprocal fashion, paracrine signalling from TGF-β-activated lung fibroblasts or IPF fibroblasts induced RAS activation in ATII cells, at least partially via the secreted protein, SPARC. Together these data identify that aberrant bi-directional epithelial-mesenchymal crosstalk in IPF drives a chronic feedback loop that maintains a wound-healing phenotype and provides self-sustaining pro-fibrotic signals.

2021-08-19 | PXD025821 | Pride

Impact of DNM3OS silencing on human lung fibroblast response to TGF-β

Project description:To assess the role of the lncRNA DNM3OS in TGF-β-induced lung fibroblast activation, we transfected in human MRC5 pulmonary fibroblasts (CCL-171) a gapmer designed against DNM3OS for 48h and then exposed these cells to TGF- β (10 ng/mL). RNA samples were harvested 24 hours after TGF-β exposition. Two independent experiments were carried out.

2019-04-11 | GSE97823 | GEO

TGF-β signature of MRC-5 fibroblasts

Project description:Human MRC5 pulmonary fibroblasts (CCL-171) were exposed or not to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Two independent experiments were carried out.

2019-04-11 | GSE97833 | GEO

Expression data from cultured human dermal fibroblasts

Project description:We used microarrays to identify the molecular targets of a novel small compound HSc025. Fibroblasts were exposed to four different stimulants, DMSO, HSc025, TGF-bata or HSc025 plus TGF-beta.

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Impact of PRRX1 knockdown on the transcriptome of normal human lung fibroblasts (NHLF) in the presence or the absence of TGF-β

Project description:To assess the role of PRRX1 in TGF-β-induced lung fibroblast activation, we transfected in human NHLF with PRRX1 or control siRNAs and then exposed these cells to TGF- β (1 ng/mL). RNA samples were harvested 48 hours after TGF-β exposition. Two independent experiments were carried out.

2023-06-20 | GSE161364 | GEO

To examine the expression of Sulf1 and Sulf2, as well as other glycan-related genes, in human Idiopathic pulmonary fibrosis (IPF) lungs compared to normal lung samples

Project description:Microarray analysis to examine glycan-related gene expression in idiopathic pulmonary fibrosis Heparan sulfate 6-O-endosulfatases (Sulf1 and Sulf2) remove 6-O sulfate groups from heparan sulfate intra-chain sites on the cell surface and in the extracellular matrix, and modulate the functions of many growth factors and morphogens including FGF, Wnt and TGF-beta. Works from our laboratory have shown that TGF-beta 1 induces Sulf1 and Sulf2 expression in a cell-type specific manner in the lung, specifically Sulf1 in lung fibroblasts and Sulf2 in type II alveolar epithelial cells. Interestingly TGF-beta 1-induced Sulf1 and Sulf2 in turn modulate TGF-beta 1 function in culture. The aim of this study is to examine the expression of Sulf1 and Sulf2 as well as other glycan-related genes (heparan biosynthetic enzymes, TGF-beta, FGF and Wnt signaling pathway components) in human idiopathic pulmonary fibrosis (IPF) lungs compared to normal lung samples. We will examine gene expression in triplicate samples from RNA of total lung homogenates from IPF and control (normal) lungs

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Effects of CDK7 inhibition on fibrotic gene expression

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2024-11-06 | GSE277437 | GEO

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