Project description:Botanic drug CA regulated the miRNAs expression in tumor cells. We used microarrays to detail the differentiation miRNAs expression in Huh7 cells treated with CA or not.
Project description:Effects of TRPC1 silencing on whole-transcriptome gene expression were determined in Huh7 hepatocellular carcinoma cells using whole-transcriptome gene expression profiling.
Project description:Our previous study has showed that complex of NF90 and nuclear factor 45 (NF45) (NF90-NF45) inhibits miRNA biogenesis through negative regulation of primary-miRNA processing step. On the other hand, miRNAs, the biogenesis of which is regulated by NF90-NF45 in hepatocellular carcinoma, are not clear. Thus, to identify the miRNAs, we performed a miRNA array using RNAs extracted from control Huh7 cells and the cells depleted of NF90. Comparison of miRNA expression profile in one non-targeting control siRNA (siNTC)- or two independent siNF90-treated Huh7 cells.
Project description:To investigate the significance of CD44+ hepatocellular carcinoma (HCC) HuH7 cells, microRNA (miRNA) expression profiles of CD44-positive HuH7, CD44-negative HuH7, and human nomal hepatocyes were analyzed. Results provide the insight into the significance of CD44-positive HCC cells as the liver CSCs.
Project description:Our previous study has showed that complex of NF90 and nuclear factor 45 (NF45) (NF90-NF45) inhibits miRNA biogenesis through negative regulation of primary-miRNA processing step. On the other hand, miRNAs, the biogenesis of which is regulated by NF90-NF45 in hepatocellular carcinoma, are not clear. Thus, to identify the miRNAs, we performed a miRNA array using RNAs extracted from control Huh7 cells and the cells depleted of NF90.
Project description:We used microarray to determine the miRNA whose expression was changed at 12 hours after Y27632 treatment Two-condition experiment; Huh7 control vs Huh7 treated with Y27632 for 12 hours
Project description:To study the role of Dengue virus serotype 4 NS1 in modulation of host cell transcriptome we performed gene expression profiling using data obtained from RNA- sequencing of total RNA from 3 different samples: Huh7 cells (no transfection), Huh7 cells transfected with pTracer-SV40 empty vector and Huh7 cells transfected with pTracer-SV40 encoding dengue virus serotype 4 NS1 (pDENV4 NS1).
Project description:To identify genes regulated by STYK1 in hepatocellular carcinoma, we performed comprehensive analyses of mRNA expression in Huh7 cells overexpressed STYK1.
Project description:Analysis of the transcriptome of L02 cells upon Huh7-cell-derived large extracellular vesicles (lEVs) incubation. L02 cells were incubated with or without lEVs (10 μg/ml) derived from control or ACSL4 knocking down (ACSL4 KD) Huh7 cells for 3 days, and the gene expression profiling analysis were then performed. The result provides a new perspective on the crosstalk between Huh7 cells and L02 cells via lEV-mediated ACSL4 transmission.