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Spodoptera frugiperda

ABSTRACT: De novo sequencing of transcriptome of Sf9 cells after Bm-15 overexpression and repression

PROVIDER: PRJNA384239 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5481197_1.fastq.gz	Fastqsanger.gz
	SRR5481197_2.fastq.gz	Fastqsanger.gz
	SRR5481198_1.fastq.gz	Fastqsanger.gz
	SRR5481198_2.fastq.gz	Fastqsanger.gz
	SRR5481199_1.fastq.gz	Fastqsanger.gz

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De novo sequencing of transcriptome of Sf9 cells after Bm-15 overexpression and repression

Project description:Small nucleolar RNAs (snoRNA) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs. But we found that knock down of a C/D box snoRNA, Bm-15, can induce apoptosis of insect Spodoptera frugiperda Sf9 cells. For the genome sequence of Spodoptera frugiperda is incomplete, here with the de novo sequencing method, transcriptome of Spodoptera frugiperda cell line Sf9 were sequenced after being transfected with overexpression vector and repression probes of snoRNA Bm-15. Results showed that 21 apoptosis-related genes were up-regulated upon Bm-15 inhibition and down-regulated with Bm-15 overexpression.

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