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Rattus

ABSTRACT: spared nerve injury model of rattus comapred with sham control Raw sequence reads

PROVIDER: PRJNA384664 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5487450_1.fastq.gz	Fastqsanger.gz
	SRR5487450_2.fastq.gz	Fastqsanger.gz
	SRR5487443_1.fastq.gz	Fastqsanger.gz
	SRR5487443_2.fastq.gz	Fastqsanger.gz
	SRR5500420_1.fastq.gz	Fastqsanger.gz

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Decoding the m6A expression profiling of spinal cord after spared nerve injury at three time points with RIP-Seq

Project description:We performed gene expression profiling analysis using data obtained from RNA-seq and m6A peaks profiling analysis using data obtained from RIP-Seq of spared nerve injury model at 3rd, 7th, 14th postoperative day and sham group. To investigate the differential m6A expression profiling, we established spared nerve injury model at 3rd, 7th, 14th postoperative day and sham group.

2022-05-06 | GSE202166 | GEO

rat T Cells: Sham vs. SNI

Project description:DNA methylation profiles of T cells comparing 9 months post Spared Nerve Injury (SNI) rats with Sham rats.

2015-10-01 | GSE70007 | GEO

rat PFC: Sham vs. SNI

Project description:DNA methylation profiles of prefrontal cortec (PFC) comparing 9 months post Spared Nerve Injury (SNI) rats with Sham rats.

2015-10-01 | GSE70006 | GEO

Small extracellular vesicles from spared nerve injury model and sham control mice differentially regulate gene expression in primary microglia

Project description:Nerve injury outcomes might be predicted by examining small extracellular vesicles (sEVs) in circulation, as their biomolecular cargo facilitates cellular communication and can alter transcriptional state and behavior of recipient cells. We found that sEVs from the serum of spared nerve injury (SNI) model male mice had seven differentially expressed miRNAs compared to sEVs from sham-operated control mice four weeks post-surgery. We investigated how these sEVs alter transcription in primary cortical microglia, a crucial mediator of neuropathic pain, using RNA sequencing. While the uptake of sEVs from both SNI model and sham groups changed gene expression in microglia compared to PBS treatment, sEVs from the sham group induced a more drastic change, particularly in genes involved in immune response. This was recapitulated by increased levels of pro-inflammatory cytokines and chemokines in microglia incubated with sEVs from sham control compared to sEVs from SNI model, naïve mice, or PBS. However, treating microglia with sEVs from female mice showed that serum sEVs derived from female SNI mice but not from female sham mice induced a more pronounced microglial secretion of pro-inflammatory mediators. Our data demonstrate that the molecular changes induced by sham surgery injuring skin and muscles are reflected in circulating sEVs in male mice four weeks later. Thus, when using sEVs from sham mice as control in comparative mechanistic studies after nerve injury, sex of mice should be taken into consideration.

2023-06-27 | GSE228065 | GEO

Small extracellular vesicles from spared nerve injury model and sham control mice differentially regulate gene expression in primary microglia

Project description:Small extracellular vesicles from spared nerve injury model and sham control mice differentially regulate gene expression in primary microglia

| PRJNA947973 | ENA

Single cell RNA-seq of the spinal cord in a neuropathic pain model.

Project description:We produced single-cell transcriptomes from the mouse spinal cord using Drop-seq in animals modeling neuropathic pain and superficial injury (SI) controls. We used the spared nerve injury (SNI) model of neuropathic pain. ~10,000 cells from sham surgery controls and ~9,000 cells from SNI animals were sequenced. Unbiased cell clustering yielded 66 spinal cell subtypes sequenced at relatively low depth (~1200 transcripts/cell). Comparisons between SI and SNI cells were performed to investigate cell-specific differences during a neuropathic pain state.

2021-03-04 | GSE134003 | GEO

Sigma-1 Receptors Control Neuropathic Pain and Peripheral Neuroinflammation After Nerve Injury in Female Mice: A Transcriptomic Study

Project description:To investigate transcriptional changes in the DRG and dorsal spinal cord in wild-type and sigma-1 knockout mice in a well-established model of neuropathic pain (Spared Nerve Injury)

2024-08-20 | GSE241361 | GEO

Rattus rattus

Project description:Rattus rattus Raw sequence reads

| PRJNA1018601 | ENA

Rattus rattus

Project description:Rattus rattus Raw sequence reads

| PRJNA344902 | ENA

G9a is essential for epigenetic silencing of K+ channel genes in acute-to-chronic pain transition

Project description:AIM: We performed RNA-sequencing experiments seeking genes whose expression changed due to nerve injury. In addition, we wanted to test whether inhibition of the methyl transferase G9a/GLP, that methylates H3K9me2, could reverse those expression changes due to nerve ligation. G9a/GLP methylase was pharmacologically inhibited using UNC0638. METHOD: We generated cDNA libraries from RNA purified from DRGs obtained from Sham operated (4), SNL (4), and SNL plus UNC0638 (3) rats. We sequenced the cDNA libraries generating single end 50 bp reads on the illumina HiSeq 2500 platform. Sequencing reads were aligned to the rat genome rn4 using TopHat RESULTS: We were able to map 16876 genes, from which 2035 changed their expression values at least two fold when we compared SNL to Sham operated (pâ?¤ 0.01). There were 1205 upregulated and 832 down-regulated genes. Next, we focused on genes whose expression was either up or down-regulated 2 fold due to nerve ligation, and the values would be normalized to control level after G9a/GLP inhibito (UNC0638)r treatment. We identified 639 genes in our data set that behave this way We generated cDNA libraries from RNA purified from DRGs obtained from Sham operated (4), SNL (4), and SNL plus UNC0638 (3) rats. We sequenced the cDNA libraries generating single end 50 bp reads on the illumina HiSeq 2500 platform. Sequencing reads were aligned to the rat genome rn4 using TopHat

2015-11-02 | E-GEOD-59043 | biostudies-arrayexpress

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