Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of IFNα Stimulated Transcriptomes in HepG2, SETD2-KO, STAT1-KO and STAT1-K525ARe HepG2 cells

PROVIDER: PRJNA384926 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5488350.fastq.gz	Fastqsanger.gz
	SRR5488351.fastq.gz	Fastqsanger.gz
	SRR5488352.fastq.gz	Fastqsanger.gz
	SRR5488353.fastq.gz	Fastqsanger.gz
	SRR5488354.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Next Generation Sequencing Facilitates Quantitative Analysis of IFNα Stimulated Transcriptomes in HepG2, SETD2-KO, STAT1-KO and STAT1-K525ARe HepG2 cells

Project description:Purpose: Using RNA-seq to analyze the different expressions induced by IFNα among the SETD2-KO HepG2 cells, STAT1-KO HepG2 cells and STAT1-K525A-Re HepG2 cells and HepG2 control cells Methods: mRNA profiles of HepG2 control cells and SETD2-KO cells, STAT1-KO cells, STAT1-K525A-Re cells were generated by deep sequencing, using BGISEQ-500RS. The sequence reads that passed quality filters were analyzed at the transcriptional level with RSEM (RNA-seq by Expectation Maximization).

2019-03-12 | GSE98372 | GEO

ISGF3 and STAT2/IRF9 direct basal and IFN-induced transcription through genome-wide binding of phosphorylated and unphosphorylated complexes to commonly ISRE containing ISGs  [ChIP-Seq]

Project description:To further understand the role of phosphorylation in ISGF3- and STAT2/IRF9-mediated constitutive and long-term IFN-I-stimulated transcriptional responses, we performed RNA-Seq and ChIP-Seq, in combination with phosphorylation inhibition and anti-viral experiments. First, we identified a group of ISRE-containing ISGs that were commonly regulated in IFNα treated WT and STAT1-KO cells. Thus, in 2fTGH and Huh7.5 WT cells IFNα-inducible transcription and anti-viral activity relied on the recruitment of the ISGF3 components STAT1, STAT2 and IRF9 in a phosphorylation- and time-dependent manner. Likewise, in ST2-U3C and Huh-STAT1KO cells lacking STAT1, ISG expression correlated with DNA-binding of phosphorylated STAT2/IRF9. This pointed to a dominant role of classical ISGF3 and STAT2/IRF9, and not U-ISGF3 or U-STAT2/IRF9, in the regulation of early and prolonged ISG expression and viral protection, in WT and STAT1-KO cells. In addition, comparative experiments in U3C (STAT1-KO) cells overexpressing all ISGF3 components (ST1-ST2-IRF9-U3C), revealed a threshold-dependent role of U-ISFG3, and potentially U-STAT2/IRF9, in the regulation of constitutive and possibly long-term IFNα-treated ISG expression and anti-viral activity.

2023-11-15 | GSE247724 | GEO

ISGF3 and STAT2/IRF9 direct basal and IFN-induced transcription through genome-wide binding of phosphorylated and unphosphorylated complexes to commonly ISRE containing ISGs  [RNA-Seq]

2023-11-15 | GSE247723 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

2021-02-27 | GSE164382 | GEO

Next-Generation Sequencing Facilitates Quantitative Analysis of Interferon-Stimulated MEFs Transcriptomes [RNA-Seq]

Project description:Next-Generation Sequencing Facilitates Quantitative Analysis of Interferon-Stimulated MEFs Transcriptomes [RNA-Seq]

| PRJNA730022 | ENA

Mus musculus

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of interferon stimulated MEFs Transcriptomes [RNA-Seq]

| PRJNA351861 | ENA

Effects of pE66L missing on ASFV

Project description:Identify the function of pE66L Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Nrl-- Retinal Transcriptomes

2020-09-23 | GSE158335 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of MYC+ and MYC+ SETD2-/- Renal Tubular Cells Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of MYC+ and MYC+ SETD2-/- Renal Tubular Cells Transcriptomes

| PRJNA516015 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of CON and PMB treatment HepG2 cells Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of CON and PMB treatment HepG2 cells Transcriptomes

| PRJNA770983 | ENA

Next-generation sequencing facilitates quantitative analysis of CON and PMB treatment HepG2 cells transcriptomes

Project description:Next-generation sequencing facilitates quantitative analysis of CON and PMB treatment HepG2 cells transcriptomes

| PRJNA856662 | ENA