Project description:Investigating the effect of Tax protein on the expression of genes in Jurkat cells. Current study will help us to elucidate the differential gene expression profiles of Tax positive Jurkat cells.
Project description:Investigating the effect of Tax protein on the expression of genes in Jurkat cells. Current study will help us to elucidate the differential gene expression profiles of Tax positive Jurkat cells. The Tax-positive (TaxP) and Tax negative (TaxN) subline of Jurkat were established. mRNA isolated from TaxN or TaxP cell lines were analyzed using gene array.
Project description:The Tesi system allows analysis of HTLV-1 Tax's impact on the transcriptome of a human CD4+ T-cell which is not derived from leukemia but directly from normal human lymphocytes. By comparing cells with and without Tax, one can specifically filter for celluar genes that are either activated or repressed in the presence of Tax. Keywords: cell type comparison, Tax