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Vernicia fordii

ABSTRACT: Flower development and sex determination in Vernicia fordii

PROVIDER: PRJNA385776 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5515193_1.fastq.gz	Fastqsanger.gz
	SRR5515193_2.fastq.gz	Fastqsanger.gz
	SRR5515194_1.fastq.gz	Fastqsanger.gz
	SRR5515194_2.fastq.gz	Fastqsanger.gz
	SRR5515195_1.fastq.gz	Fastqsanger.gz

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Flower development and sex determination in Vernicia fordii

Project description:To further elucidate the molecular mechanism underling sex determination at the divergence stage of male and female flowers, the comparative transcriptome analysis was performed. In total, 56,065 unigenes were generated 24,567 transcripts were identified. Among 608 differential expression genes (DEGs), 310 DEGs showed significant expression in males and 298 DEGs in females. The data showed that the sexual dimorphism of female flowers was affected by jasmonic acid, transcription factors and some genes related with activity of floral meristem, which were considered as the candidate sex determination genes. In this study, interesting information will be provided in understanding the development of unisexual flower and the regulatory networks hidden the sex determination in V. fordii, which is useful for the practice of improving its yield.

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Identification of microRNAs and their targets in Vernicia fordii seeds by high-throughput sequencing

Project description:The tung (Vernicia fordii) oil is a valuable industrial oil and has been considered to the production of biodiesel. Because of the poor agronomic traits of this crop, efforts have been made to transfer genes related to the production of its oil to higher yielding plants. However, the mechanisms highlighting gene expression in this plant is poorly understood. MicroRNAs are endogenously encoded small RNAs that play a key role in diverse plant biological processes. In order to identify and characterize miRNAs and their targets in V. fordii we generated by deep sequencing a sRNA and a mRNA library from mature seeds. Using sequence homology we identified 138 conserved plant miRNAs, which were distributed in 34 conserved miRNAs families. Further computation analysis allowed the prediction of secondary structures for 18 conserved and 14 novel miRNAs, as well as several miRNA variants (isomiRNAs). The predicted miRNA target genes are involved in a broad range of physiological functions, including growth and development, stress resistance and also lipid metabolism. This study presents the first identification of V. fordii miRNAs and contributes to understand the function of these miRNAs.

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