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Transcriptional Regulation by NFATc1 in CD8+ T cells

ABSTRACT: Transcriptional Regulation by NFATc1 in CD8+ T cells

PROVIDER: PRJNA386031 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5515785.fastq.gz	Fastqsanger.gz
	SRR5515786.fastq.gz	Fastqsanger.gz
	SRR5520190.fastq.gz	Fastqsanger.gz
	SRR5520191.fastq.gz	Fastqsanger.gz
	SRR5520192.fastq.gz	Fastqsanger.gz

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Transcriptional Regulation by NFATc1 in CD8+ T cells [RNA-seq]

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NFATc1 drives allergic contact dermatitis reponses by controlling the induction of IL-17-producing CD8 cells

Project description:A plethora of data supports a major role of CD4+ and CD8+ T lymphocytes for the initiation, progression and maintenance of allergic contact dermatitis (ACD). However, in-depth understanding of the underlying molecular mechanisms is still limited. NFATc1 , a central component of the Ca++-Calcineurin-NFAT-signalling network, plays an essential role in T cell activation. We therefore investigated its impact on contact hypersensitivity (CHS), the mouse model for ACD. The CHS response to 2,4,6-trinitrochlorobenzene (TNCB) was diminished in Nfatc1fl/flxCd4-cre mice (Nfatc1-/-) as compared to wild-type (WT) animals and associated with a lower percentage of interleukin (IL)17-producing CD8+T (Tc17) cells in both inflamed skin and draining lymph nodes (dLN). In vitro Tc17 polarization assays revealed that Nfatc1-/- CD8+ T cells have a reduced capacity to polarize into Tc17 cells. Applying single-cell RNA sequencing, we realized that NFATc1 controls the T cell differentiation fate. In the absence of NFATc1, CD8+ T cells favour the development of Interferon (IFN)-g-secreting CD8+ T (Tc1) lymphocytes while in its presence they turn into Tc17 cells. Finally, we showed the adoptive transfer of TNCB-sensitized WT CD8+T cells rescued tThe CHS response could be rescued in naïve Nfatc1-/-mice by adoptive transfer of TNCB-sensitized WT CD8+T cells. Our data demonstrate that NFATc1 acts as a molecular switch controllcontrolsing the development of Tc17 cells and can be used as a target to alleviate surveilling CD8+T cell-mediated CHS responses.

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Transcriptional Regulation by NFATc1 during murine thymocyte development [ChIP-seq]

Project description:Transcriptional Regulation by NFATc1 during murine thymocyte development [ChIP-seq]

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Transcriptional Regulation by NFATc1 during murine thymocyte development [gammadelta_T_cells RNA-seq]

Project description:Transcriptional Regulation by NFATc1 during murine thymocyte development [gammadelta_T_cells RNA-seq]

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Transcriptional Regulation by NFATc1 during murine thymocyte development [BirA RNA-seq]

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Transcriptional Regulation by NFATc1 during murine thymocyte development [ChIP-seq]

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